Chlorogenic Acid

1. English Title: Chlorogenic Acid
2. Specification

• – Chlorogenic Acid ≥ 98% (HPLC)
• – Water-soluble Powder: Chlorogenic Acid ≥ 95% (HPLC), particle dimension ≤5μm (enhanced solubility by way of microencapsulation)

1. Look

• – Powder: Mild yellow to brownish-yellow crystalline powder, odorless, barely bitter
• – Liquid: Darkish brown clear answer (20% w/v in ethanol-water combination)

1. CAS NO.：327-97-9
2. Lead Time: 3-7 Working Days
3. Bundle

• – Powder: 1kg/aluminum foil bag, 25kg/drum (double-layer PE bag inside cardboard drum)
• – Liquid: 20L/food-grade HDPE drum

1. Foremost Market: World (North America, Europe, Asia-Pacific), The main market is within the useful meals and dietary complement sectors
2. Software Eventualities

Core Properties

• Molecular Formulation: C₁₆H₁₈O₉
• Solubility: Soluble in water (5g/L at 25°C), ethanol, and acetone; water-soluble formulations enhance bioavailability in drinks
• Key Actions: Antioxidant (ORAC worth: ~5000 μmol/g), anti-inflammatory, anti-obesity, glucose-lowering

Software Eventualities

1. Purposeful Meals & Drinks

• Natural Antioxidant:
• – Added to juices, power drinks, and plant-based milks at 0.1-0.5% to increase shelf life and improve dietary worth.
• – Widespread in “clear label” merchandise as a substitute for artificial preservatives (e.g., BHA/BHT).
• Metabolic Well being Drinks:
• – Formulated in weight administration drinks (e.g., inexperienced espresso extract blends) to inhibit carbohydrate absorption and increase fats oxidation.

2. Dietary Dietary supplements

• Blood Sugar Management:
• – Capsules (200-500mg/day) for kind 2 diabetes help, inhibiting α-glucosidase to sluggish sugar digestion.
• Liver Well being Formulation:
• – Mixed with milk thistle extract (silymarin) to guard hepatocytes from oxidative harm in liver-care dietary supplements.

3. Cosmetics & Private Care

• Antioxidant Skincare:
• – Serums (1-3% focus) to neutralize UV-induced free radicals, lowering hyperpigmentation and nice traces.
• Hair Care:
• – Shampoos and scalp therapies to fight dandruff and promote follicle well being by way of anti-inflammatory results.

4. Prescription drugs & Biotechnology

• Medical Analysis:
• – Investigated for treating metabolic syndrome (e.g., pre-diabetes) and non-alcoholic fatty liver illness (NAFLD).
• Drug Supply Methods:
• – Liposomal chlorogenic acid formulations for enhanced oral bioavailability (will increase absorption by 30% vs. normal powder).

5. Animal Diet

• Poultry & Aquaculture:
• – Feed components (50-200ppm) to enhance progress efficiency and scale back oxidative stress in high-density farming.
• Pet Meals:
• – Senior pet formulation to help joint well being and cognitive operate by way of anti-inflammatory exercise.

Detection Strategies

• Excessive-Efficiency Liquid Chromatography (HPLC):
• – Column: C18 (250×4.6mm, 5μm), Cellular Section: Acetonitrile-0.1% phosphoric acid (20:80 v/v), Move: 1.0mL/min, Detection: 327nm.
• Mass Spectrometry (MS):
• – Confirms molecular ion (m/z 353.1 for [M-H]⁻) and distinguishes isomers (e.g., caffeoylquinic acid isomers).
• ORAC Assay:
• – Measures antioxidant capability towards peroxyl radicals, reported as μmol Trolox equivalents/g.

Supply & Benefits

• Natural Supply: Extracted from inexperienced espresso beans (5-10% content material), sunflower seeds, and Eucommia ulmoides leaves by way of ethanol/water extraction.
• Sustainability: Espresso cherry by-products (e.g., pulp) function a renewable supply, lowering waste in espresso processing.
• Bioactivity Synergy: Usually co-extracted with different polyphenols (e.g., caffeine, quercetin) for enhanced well being advantages.

Regulatory Compliance

• GRAS Standing: Accepted as a dietary ingredient within the US (2015).
• EU Novel Meals Regulation: Notified to be used in useful meals (e.g., sugar-free drinks) at ≤0.3% focus.
• China NMPA: Listed as a permitted meals additive (antioxidant, E quantity: INS 327 equal).

Classes: Functional Foods, Beverage addition, Dietary supplements, Food additives, Functional food

Chlorogenic Acid: Nature’s Potent Antioxidant | Premium Provider Highlight

1. What is Chlorogenic Acid (CGA)?

Chlorogenic Acid (CGA), chemically generally known as 5-O-caffeoylquinic acid (5-CQA), is a pivotal bioactive polyphenol and ester fashioned between caffeic acid and quinic acid. Functioning as a significant secondary plant metabolite, it acts as a sturdy antioxidant and performs vital roles in plant protection mechanisms. Its prominence lies in its abundance inside particular botanicals and its wide-ranging physiological results in people, making it a focus in nutraceutical, pharmaceutical, and beauty analysis.

2. Product Supply, Chemical Properties & Key Identifiers

• Main Natural Sources: Inexperienced espresso beans (Coffea spp.) are the richest pure reservoir, particularly earlier than roasting which degrades CGA. Fruit and vegetable Important portions are additionally present in sunflower seeds, artichokes, eucommia bark, apples, pears, berries (like blueberries), potatoes, and tobacco leaves.
• Chemical Properties:
  • Look: Sometimes a white to mild yellow crystalline powder.
  • Solubility: Soluble in polar solvents like ethanol, methanol, acetone, and scorching water; barely soluble in chilly water; insoluble in non-polar solvents.
  • Stability: Delicate to warmth, mild, alkaline situations, and oxidation. Optimum storage requires cool, darkish, dry situations.
• Key Identifiers:
  • CAS Quantity: 327-97-9 (frequent for 5-CQA, the predominant isomer)
  • Molecular Formulation (MF): C₁₆H₁₈O₉
  • Molecular Weight (MW): 354.31 g/mol

3. Efficiency, Well being Advantages, Dosage & Security

• “Greatest” Supply & Highest Focus: Unroasted inexperienced espresso beans (Coffea arabica / Coffea canephora) are unequivocally the richest pure supply, usually containing 5-12% chlorogenic acids by dry weight, with 5-CQA being the dominant isomer (typically 40-60% of whole CGAs). Specialty extracts can focus CGA ranges considerably greater (e.g., 40%, 50%, 70%+).
• Well being Advantages & Physiological Results:
  • Potent Antioxidant: Neutralizes free radicals (ROS/RNS), lowering oxidative stress linked to persistent illnesses and getting older. Modulates Nrf2 pathway.
  • Metabolic Well being: Influences glucose metabolism by inhibiting glucose-6-phosphatase and modulating glucose absorption; improves insulin sensitivity. Linked to weight administration help.
  • Cardiovascular Assist: Could assist decrease blood strain (ACE inhibition), enhance endothelial operate, and positively impression lipid profiles.
  • Neuroprotective Potential: Crosses BBB; reveals anti-inflammatory and antioxidant results in neural tissues; potential in mitigating neurodegenerative processes.
  • Hepatoprotective Results: Helps liver well being by lowering oxidative stress and irritation.
  • Anti-inflammatory Motion: Modulates NF-κB and different inflammatory signaling pathways.
• Each day Consumption: No universally established RDI. Widespread supplemental doses vary from 120mg to 300mg of CGA, typically derived from 300mg to 600mg of inexperienced espresso bean extract (standardized to 40-50% CGA). At all times seek the advice of a healthcare supplier earlier than beginning supplementation.
• Precautions & Aspect Results:
  • Usually Acknowledged as Protected (GRAS) at typical dietary/supplemental ranges.
  • Potential delicate unintended effects: Gastrointestinal upset (nausea, diarrhea), jitteriness (particularly if delicate to caffeine, although CGA itself will not be a stimulant, inexperienced espresso extracts comprise low caffeine).
  • Necessary Interactions: Could potentiate blood strain medicines (threat of hypotension) and diabetes medicines (threat of hypoglycemia). Seek the advice of physician if taking these.
  • Being pregnant/Breastfeeding: Security not absolutely established; keep away from supplementation except suggested by doctor.

1. Premium Supply: Shaanxi Zhonghong Investment Technology Co., Ltd.

As a pacesetter in bioactive compound innovation for 28 years, Shaanxi Zhonghong Investment Technology Co., Ltd. stands on the forefront of the pure extract trade. This high-tech enterprise integrates agile R\&D, collaborative innovation, precision manufacturing, and international distribution, specializing within the superior extraction, purification, and software of potent plant constituents.

• Core Experience: Superior processing of Natural Plant Extracts, Beauty Actives, Nutraceutical & Pharmaceutical Substances, Natural Pigments, and Plant-Based mostly Sweeteners.
• Scientific Prowess: 5 university-affiliated joint laboratories foster cutting-edge analysis. Proprietary methodologies protected by 20+ patents and a globally unique compound library guarantee technological superiority.
• Unmatched High quality Infrastructure: Funding in HPLC, UPLC, GC-MS, and Superconducting NMR ensures analytical precision. Our stringent protocols implement purity requirements exceeding trade norms by 20%+.
• World Provide Chain: Trusted companion offering bespoke ingredient options to multinational pharmaceutical firms, analysis establishments, and producers throughout 80+ nations in Asia, Europe, and the Americas.

1. Chlorogenic Acid Advantages: Deep Dive into Well being Impacts

Past its foundational antioxidant position, CGA exerts multifaceted organic results:

• Glucose Homeostasis: Inhibits alpha-glucosidase and alpha-amylase enzymes within the intestine, slowing carbohydrate breakdown and glucose absorption. Enhances AMPK activation and GLUT4 translocation in peripheral tissues, bettering glucose uptake. Modulates hepatic gluconeogenesis.
• Lipid Metabolism: Could scale back hepatic triglyceride synthesis and improve fatty acid beta-oxidation, contributing to improved lipid profiles.
• Vascular Well being: Promotes nitric oxide (NO) bioavailability, resulting in vasodilation and lowered peripheral resistance. Reveals anti-atherogenic properties.
• Mitochondrial Biogenesis: Rising proof suggests CGA might activate PGC-1α, enhancing mitochondrial operate and mobile power metabolism.
• Intestine Microbiota Modulation: Serves as a substrate for useful intestine micro organism, selling a more healthy microbiome profile and manufacturing of bioactive metabolites like butyrate.

1. Chlorogenic Acid: Utilization Tips

• Timing: Usually taken 30-60 minutes earlier than predominant meals to modulate glucose and fats absorption.
• Bioavailability Enhancement: Combining with piperine (black pepper extract) might enhance absorption, though analysis is ongoing. Formulations using phospholipids or cyclodextrins are additionally explored.
• Synergistic Formulations: Steadily mixed with different bioactive compounds like chromium picolinate, berberine, or gymnema sylvestre for enhanced metabolic help.

1. Chlorogenic Acid Product Specs (Exemplary)

Shaanxi Zhonghong adheres to the strictest worldwide high quality requirements for Chlorogenic Acid.

1. Sourcing: World procurement of premium, non-GMO inexperienced espresso beans.
2. Pre-treatment: Cleansing, milling, and defatting (utilizing supercritical CO₂ or food-grade solvents).
3. Extraction: Multi-stage counter-current extraction utilizing hydro-ethanolic options beneath optimized temperature/pH management. Various strategies embody ultrasonic or microwave-assisted extraction (UAE/MAE).
4. Main Purification: Macroporous resin adsorption/desorption for gross impurity removing and preliminary focus.
5. Excessive-Decision Chromatography: Proprietary preparative HPLC or simulated shifting mattress (SMB) chromatography for exact isolation of 5-CQA and different isomers, attaining ultra-high purity (>98%).

Notice: Specs are topic to customization per buyer necessities and regulatory jurisdiction (FDA, EFSA, JP, and so forth.).

8. Superior Manufacturing Course of

Shaanxi Zhonghong employs a classy, vertically built-in manufacturing chain:

1. Sourcing: World procurement of premium, non-GMO inexperienced espresso beans.
2. Pre-treatment: Cleansing, milling, and defatting (utilizing supercritical CO₂ or food-grade solvents).
3. Extraction: Multi-stage counter-current extraction utilizing hydro-ethanolic options beneath optimized temperature/pH management. Various strategies embody ultrasonic or microwave-assisted extraction (UAE/MAE).
4. Main Purification: Macroporous resin adsorption/desorption for gross impurity removing and preliminary focus.
5. Excessive-Decision Chromatography: Proprietary preparative HPLC or simulated shifting mattress (SMB) chromatography for exact isolation of 5-CQA and different isomers, attaining ultra-high purity (>98%).
6. Focus: Low-temperature vacuum evaporation.
7. Crystallization & Isolation: Managed crystallization processes.
8. Drying: Lyophilization (freeze-drying) or mild spray drying to protect stability.
9. Mixing & Standardization: Exact adjustment to assured efficiency specs.
10. Various Software Eventualities

• Nutraceuticals & Dietary Dietary supplements: Weight administration formulation, blood sugar help complexes, antioxidant blends, power/metabolism boosters (capsules, tablets, powders).
• Purposeful Meals & Drinks: Fortified juices, power drinks, sports activities diet bars, wholesome snacks, baked items (requires stability options).
• Cosmeceuticals: Anti-aging serums/lotions (combats oxidative stress & photoaging), brightening merchandise (inhibits tyrosinase), soothing lotions (anti-inflammatory).
• Prescription drugs: Analysis into formulations for metabolic syndrome, cardiovascular help, and neuroprotection.
• Animal Diet: Pet well being dietary supplements, livestock efficiency enhancers (antioxidant help).

1. Rigorous High quality Management (QC)

High quality is paramount at Shaanxi Zhonghong. Our QC system implements cGMP and ISO requirements:

• Incoming Uncooked Materials Inspection: Full identification, purity, and contaminant testing towards strict specs.
• In-Course of Controls (IPC): Actual-time monitoring of vital parameters (pH, temp, focus, solvent residues) at each manufacturing stage utilizing validated strategies.
• Stability Research: Accelerated and long-term stability testing (ICH Q1A(R2)) to find out shelf-life and optimum storage situations.
• Superior Analytical Testing: State-of-the-art instrumentation (HPLC-DAD/ELSD/CAD, UPLC-QTOF-MS, GC-MS, ICP-MS, NMR) for complete characterization (assay, impurities, isomers), heavy metals, pesticide residues, and microbiological security.
• Methodology Validation: All analytical strategies endure full validation per ICH Q2(R1) pointers (specificity, accuracy, precision, linearity, vary, LOD/LOQ, robustness).
• Traceability & Documentation: Full batch traceability from uncooked materials to completed product. Complete documentation making certain information integrity (ALCOA+ ideas).
• Third-Social gathering Verification: Common audits and collaborative testing with accredited impartial laboratories.

1. Safe Packaging & World Logistics

• Main Packaging: Meals-grade polyethylene liners inside multi-wall kraft paper luggage (25kg). Choices embody vacuum-sealed foil luggage (1kg, 5kg) or HDPE drums for enhanced moisture/oxygen barrier.
• Secondary Packaging: Strong corrugated export cartons or fiber drums.
• Storage: Strictly in a cool (\<25°C), dry (RH\<60%), darkish, well-ventilated warehouse.
• Logistics: Experience in international transport by way of air, sea, or land freight. Complete documentation (CoA, CoC, MSDS, Certificates of Origin). Compliance with worldwide laws (REACH, FDA, FSSAI, and so forth.). Temperature-controlled choices out there.

1. Analysis Frontiers & Innovation

• Well being Mechanisms: Elucidating exact molecular targets (e.g., particular kinases, transcription components), epigenetic results, and interactions with the gut-brain axis.
• Bioavailability Enhancement: Creating novel supply programs: phospholipid complexes, cyclodextrin inclusion complexes, strong lipid nanoparticles (SLNs), nanoemulsions, self-microemulsifying drug supply programs (SMEDDS).
• Medical Purposes: Strong human trials exploring efficacy in NAFLD, neurodegenerative issues, and as an adjuvant remedy in metabolic illnesses.
• Artificial Biology: Exploring microbial manufacturing pathways (e.g., engineered E. coli, S. cerevisiae) for sustainable, high-yield CGA manufacturing.
• New Isomers & Derivatives: Investigating the bioactivity and purposes of much less frequent CGA isomers and semi-synthetic derivatives.

1. Steadily Requested Questions (FAQ)

• Q: Is Chlorogenic Acid the identical as Inexperienced Espresso Bean Extract?
  • A: No. Inexperienced Espresso Bean Extract (GCBE) comprises Chlorogenic Acids (CGAs), primarily 5-CQA. CGA is the particular lively compound, whereas GCBE is the supply extract standardized to a sure CGA share (e.g., 45%, 50%).
• Q: Does Chlorogenic Acid comprise caffeine?
  • A: Pure Chlorogenic Acid powder (like ours, >98%) comprises nearly no caffeine. Nevertheless, inexperienced espresso bean extracts standardized to CGA will comprise low ranges of caffeine (usually 1-4% or much less).
• Q: What’s the very best time to take Chlorogenic Acid?
  • A: For metabolic/glucose advantages, taking it 30-60 minutes earlier than meals is often really helpful. For common antioxidant help, timing is much less vital.
• Q: Are there any main drug interactions?
  • A: Sure. Train warning if taking blood strain remedy (threat of additive hypotension) or diabetes remedy (threat of additive hypoglycemia). At all times seek the advice of your physician earlier than use.
• Q: What is the standard shelf life?
  • A: When saved correctly (cool, dry, darkish place in sealed containers), high-purity CGA powder usually has a shelf lifetime of 24-36 months.
• Q: Is your Chlorogenic Acid appropriate for vegan/vegetarian merchandise?
  • A: Sure, our Chlorogenic Acid is 100% plant-derived and appropriate for vegan and vegetarian formulations.

1. The place to Purchase Premium Chlorogenic Acid?

Supply high-purity, reliably manufactured Chlorogenic Acid (5-CQA) immediately from the skilled:

• Contact: Mr. Liao Daohai
• E mail: liaodaohai@gmail.com
• Web site: https://www.aiherba.com (Discover our full catalog of premium botanical extracts)
• Inquire Right now: Request a complete Certificates of Evaluation (CoA), technical file, and aggressive citation to your particular software wants (analysis, nutraceutical, beauty, meals).

1. Conclusion

Chlorogenic Acid (5-CQA) stands as a scientifically validated, multi-functional phytochemical with profound antioxidant, metabolic, and health-promoting properties. Its purposes span nutraceuticals, useful meals, superior cosmetics, and pharmaceutical analysis. Shaanxi Zhonghong Investment Technology Co., Ltd. leverages 28 years of experience, cutting-edge expertise (patented chromatography, HPLC/SMB purification), and uncompromising high quality management (purity >98%, contaminants beneath stringent limits) to ship premium-grade Chlorogenic Acid to the worldwide market. Backed by college partnerships, an unlimited compound library, and a worldwide distribution community, we offer tailor-made, dependable options for trade leaders looking for the best bioactive components. Companion with us to your Chlorogenic Acid necessities.

16. References
17. Clifford, M. N. (1999). Chlorogenic acids and different cinnamates – nature, prevalence and dietary burden. Journal of the Science of Meals and Agriculture, 79(3), 362-372.
18. Tajik, N., Tajik, M., Mack, I., & Enck, P. (2017). The potential results of chlorogenic acid, the primary phenolic parts in espresso, on well being: a complete evaluate of the literature. European Journal of Diet, 56(7), 2215-2244.
19. Meng, S., et al. (2013). Roles of chlorogenic Acid on regulating glucose and lipids metabolism: a evaluate. Proof-Based mostly Complementary and Various Medication, 2013.
20. European Meals Security Authority (EFSA). (2011). Scientific Opinion on the substantiation of well being claims associated to chlorogenic acids from inexperienced espresso… EFSA Journal, 9(4), 2034.
21. Worldwide Council for Harmonisation (ICH) Tips: Q1A(R2) Stability Testing, Q2(R1) Validation of Analytical Procedures.
22. United States Pharmacopeia (USP) Normal Chapters: Microbiological Examination, Microbiological Checks, Loss on Drying, Optical Rotation, Residue on Ignition.
23. ISO Requirements (e.g., ISO 17025 for laboratory competence, ISO 22000 for meals security administration). Particular strategies (e.g., ISO strategies for pesticides, heavy metals). (Notice: Particular technique numbers rely on the analyte).