Withanolides
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Withanolides

Withanolides Extract | High-Purity Ashwagandha Active Compounds | GMP Factory & Bulk Supplier – AIHerba®

  1. English Name: Withanolides
  1. Specification
    • Total Withanolides Content: ≥ 90% (HPLC); high – purity grade: ≥ 95% (HPLC)
    • Solubility: Sparingly soluble in water; soluble in organic solvents such as ethanol, methanol, chloroform, and DMSO
    • Moisture Content: ≤ 5%
    • Heavy Metals: ≤ 10 ppm
    • Residual Solvents: ≤ 0.5%
    • Particle Size: Standard powder ≤ 100 μm; micronized powder ≤ 5 μm for better bioavailability
  1. Appearance
    • Yellow to brownish – yellow fine powder, with a mild, characteristic herbal odor
  1. CAS NO.
    • As a class, no single CAS number; individual compounds have specific numbers (e.g., Withaferin A: 5119 – 48 – 8)
  1. Lead Time: 5 – 7 Working Days
  1. Package
    • 1 kg/aluminum foil bag, 25 kg/drum (double – layer PE bag inside cardboard drum)
  1. Main Market: Global market, with significant demand in Asia (India, China), North America, and Europe, mainly in the nutraceutical, pharmaceutical, and cosmetic industries
  1. Application Scenarios

Core Properties

  • Chemical Structure:
    • Steroidal lactones derived from the Withania somnifera (Ashwagandha) plant, featuring a unique ergostane – type steroid backbone with various functional groups (hydroxyl, acetyl, and lactone rings).
  • Bioactive Features:
    • Adaptogenic Activity: Helps the body adapt to stress by modulating the hypothalamic – pituitary – adrenal (HPA) axis, reducing cortisol levels.
    • Anti – inflammatory: Inhibits NF – κB pathway and reduces production of pro – inflammatory cytokines like TNF – α and IL – 6.
    • Antioxidant: Scavenges free radicals such as superoxide and hydroxyl radicals, protecting cells from oxidative damage.
    • Immunomodulatory: Balances immune responses, enhancing immunity in healthy individuals and reducing overactive immune reactions in autoimmune conditions.
    • Neuroprotective: Protects neurons from damage, potentially beneficial for neurodegenerative diseases by reducing oxidative stress and inflammation in the brain.

Application Scenarios

1. Dietary Supplements

  • Stress & Anxiety Relief:
    • Capsules or tablets (300 – 600 mg per serving) are popular for reducing stress and improving mental well – being. Often combined with other adaptogens like Rhodiola rosea.
  • Immune Support:
    • Supplements formulated to boost immune function, especially during seasonal changes or for individuals with weakened immunity.
  • Athletic Performance:
    • Powder or liquid formulations (100 – 200 mg per serving) claimed to enhance endurance, reduce muscle fatigue, and aid recovery by modulating stress hormones and reducing inflammation.

2. Pharmaceuticals

  • Clinical Research:
    • Investigated for treating various diseases:
      • Cancer: Withaferin A, a prominent withanolide, shows potential in inhibiting tumor growth, inducing apoptosis, and preventing metastasis in pre – clinical studies.
      • Autoimmune Disorders: Studies suggest it may help manage conditions like rheumatoid arthritis by suppressing inflammation and regulating immune cells.
      • Neurological Conditions: Under exploration for treating Alzheimer’s and Parkinson’s diseases due to its neuroprotective properties.
  • Traditional Medicine:
    • Key ingredient in Ayurvedic medicine, used for centuries to treat a range of ailments including stress, insomnia, and weakness. Modern formulations standardize withanolide content for consistent efficacy.

3. Cosmetics & Personal Care

  • Anti – Aging Skincare:
    • Serums, creams, and masks (1 – 3% concentration) formulated to reduce wrinkles, improve skin elasticity, and protect against environmental damage through antioxidant and anti – inflammatory actions.
  • Hair Care:
    • Shampoos and conditioners containing withanolides aim to strengthen hair follicles, prevent hair loss, and soothe the scalp by reducing inflammation.

4. Animal Health

  • Livestock Feed Additives:
    • Poultry and livestock feed (50 – 200 ppm) to enhance growth performance, improve feed efficiency, and boost immune function in animals.
  • Pet Supplements:
    • Chewable tablets or powders for dogs and cats, especially beneficial for older pets to support joint health, reduce stress, and improve overall vitality.

Detection Methods

  • High – Performance Liquid Chromatography (HPLC):
    • Column: C18 (250 × 4.6 mm, 5 μm), Mobile Phase: Acetonitrile – 0.1% formic acid (gradient elution), Flow Rate: 1.0 mL/min, Detection Wavelength: 220 – 280 nm (varies by withanolide type).
  • Liquid Chromatography – Mass Spectrometry (LC – MS):
    • Used for identifying and quantifying individual withanolides in complex samples, providing high – sensitivity and accurate structural confirmation.
  • Nuclear Magnetic Resonance (NMR):
    • Helps in determining the chemical structure of withanolides, especially useful for novel compound identification and purity verification.

Source & Advantages

  • Natural Sourcing:
    • Primarily extracted from Withania somnifera (Ashwagandha), a herb native to India and North Africa. Also found in other Withania species.
  • Sustainable Production:
    • Ashwagandha is cultivated on a large scale, ensuring a stable supply. Modern extraction techniques like supercritical CO₂ extraction preserve bioactivity while minimizing environmental impact.
  • Regulatory Acceptance:
    • Recognized as safe in many regions:
      • US FDA: Generally regarded as safe (GRAS) for use in dietary supplements.
      • EU: Permitted as a natural ingredient in food supplements and cosmetics, subject to quality and safety standards.
Categories: , , ,

Withanolides Extract (Ashwagandha Active Compounds)

High-Purity Natural Extract | Bulk Supply | OEM & ODM Factory – AIHerba®

1. Product Overview

Withanolides are a group of naturally occurring steroidal lactones found primarily in Withania somnifera (Ashwagandha), a revered adaptogenic herb in Ayurvedic medicine. These bioactive compounds are responsible for most of Ashwagandha’s pharmacological activities, including stress reduction, cognitive enhancement, anti-inflammatory, and rejuvenating effects.

AIHerba® (Shaanxi Zhonghong Investment Technology Co., Ltd.) offers high-purity Withanolides extract standardized to 1%–10% by HPLC or UV, derived from premium roots and leaves of Withania somnifera.
We ensure consistent potency, traceable sourcing, and compliance with international quality standards through GMP-certified manufacturing and advanced extraction technologies.

2. Botanical Source & Production Origin

  • Botanical Name: Withania somnifera (L.) Dunal

  • Common Name: Ashwagandha Root Extract

  • Plant Part Used: Root and Leaf

  • Active Ingredient: Withanolides

  • Specification: 1%, 2.5%, 5%, 10% (HPLC/UV)

  • Origin: Shaanxi, China

  • CAS No.: 5119-48-2

  • Molecular Formula: C28H38O6

3. Key Benefits & Biological Activities

  1. Adaptogenic Support: Helps the body resist physical and mental stress.

  2. Cognitive Enhancement: Promotes memory, focus, and neuroprotection.

  3. Anti-Inflammatory & Antioxidant: Reduces oxidative stress and inflammation markers.

  4. Hormonal Balance: Supports testosterone levels and reproductive health.

  5. Immune System Modulation: Enhances resistance to infections and fatigue.

  6. Anti-Anxiety & Sleep Aid: Promotes calmness and quality sleep without sedation.

4. Health Benefits & Clinical Insights

  • Neuroprotective: May reduce neurodegenerative effects and improve synaptic function (PubMed: PMID 15711595)

  • Stress & Cortisol Regulation: Proven to lower serum cortisol and anxiety levels (NIH: PMID 23439798)

  • Endocrine & Metabolic Health: Improves thyroid and insulin sensitivity (PubMed: PMID 28707062)

5. Traditional Uses

Ashwagandha has been used in Ayurvedic medicine for over 3,000 years as a Rasayana (rejuvenative tonic) to promote vitality, longevity, and resistance to disease. Traditionally prescribed for:

  • Fatigue and weakness

  • Cognitive decline

  • Stress and anxiety disorders

  • Sexual dysfunction

  • Immune system support

6. Recommended Dosage & Usage Guidelines

Application Form Typical Dosage Notes
Capsules/Tablets 250–600 mg/day Standardized 2.5–5% Withanolides
Beverages/Tonics 100–200 mg/day Soluble powder recommended
Functional Foods 0.1–0.5% inclusion Stable in low-heat formulations
Cosmetics 0.1–2% topical use Skin-soothing, anti-stress, anti-aging effects

Consult a healthcare professional before use, especially for pregnant or lactating women.

7. Best Formulation Guidelines

  • Combine with L-Theanine, Magnesium, or Melatonin for relaxation blends.

  • Pair with Ginseng Extract or Rhodiola Rosea for adaptogenic synergy.

  • In skincare, use alongside Niacinamide or Aloe Vera Extract for anti-fatigue, stress-relief formulations.

8. Production Process

Step 1: Raw Material Selection (Certified Withania somnifera roots)
Step 2: Cleaning & Drying
Step 3: Water/Ethanol Extraction
Step 4: Filtration & Concentration
Step 5: Vacuum Drying & Powder Milling
Step 6: HPLC Standardization & Quality Testing
Step 7: GMP Packaging

9. Product Specifications (COA Example)

A. General Parameters

Item Specification Test Method
Appearance Brown-yellow fine powder Visual
Odor Characteristic Organoleptic
Assay (Withanolides) ≥5.0% HPLC
Moisture ≤5.0% GB 5009.3
Ash ≤5.0% GB 5009.4

B. Pesticide Residue

Pesticide Limit (mg/kg) Method
Organochlorine compounds ≤0.1 GC-MS
Organophosphorus compounds ≤0.5 GC-MS
Pyrethroids ≤0.5 GC-MS
Carbamates ≤0.5 HPLC-MS/MS

C. Heavy Metals

Element Limit (ppm) Method
Lead (Pb) ≤3.0 ICP-MS
Arsenic (As) ≤2.0 ICP-MS
Cadmium (Cd) ≤1.0 ICP-MS
Mercury (Hg) ≤0.1 ICP-MS

D. Microbiological Control

Test Specification Method
Total Plate Count ≤1,000 cfu/g GB 4789.2
Yeast & Mold ≤100 cfu/g GB 4789.15
E. coli Negative GB 4789.3
Salmonella Negative GB 4789.4
Staphylococcus aureus Negative GB 4789.10

10. Storage & Shelf Life

  • Storage: Keep sealed in a cool, dry, and well-ventilated area, away from direct light.

  • Shelf Life: 24 months in original unopened packaging.

11. Packaging, MOQ & Logistics

  • Packaging: 1kg aluminum foil bag / 25kg fiber drum

  • MOQ: 1kg (sample order available)

  • Samples: Free ≤50g for quality evaluation

  • Delivery: 3–7 working days by DHL, FedEx, or air freight

  • Trade Terms: EXW, FOB, CIF, DDP available

12. Applicable Industries & Application Scenarios

  • Dietary Supplements: Adaptogenic capsules, nootropic formulas

  • Functional Foods & Beverages: Anti-stress drinks, vitality boosters

  • Cosmetics & Personal Care: Anti-aging, anti-fatigue skincare lines

  • Pharmaceuticals: Stress relief and neuroprotective formulations

13. Certifications & Quality Assurance

  • GMP, ISO9001, HACCP Certified Factory

  • Third-Party Lab Tested (SGS, Eurofins)

  • Traceable Supply Chain Documentation

  • Compliant with USP, EP, and GB Standards

14. Factory Expertise & Client Experience

With 28+ years of production experience, AIHerba® supports global clients in OEM/ODM formulation, customized standardization, and regulatory documentation (COA, MSDS, TDS).
Our extracts are widely used by nutraceutical brands, functional beverage companies, and cosmetic manufacturers in Europe, USA, Japan, and South Korea.

15. Frequently Asked Questions (FAQ)

Q1: What’s the difference between Withanolides and Ashwagandha extract?
A: Withanolides are the active compounds in Ashwagandha; our extract is standardized for these compounds to ensure consistent potency.

Q2: Can it be used in food formulations?
A: Yes, our food-grade extract meets international food safety standards.

Q3: Is your Withanolides extract organic?
A: Organic versions are available upon request (USDA/EU certified).

Q4: What’s your minimum order quantity (MOQ)?
A: 1kg for trial orders; bulk contracts from 25kg+.

Q5: Do you provide COA and MSDS?
A: Yes, full documentation is supplied with every batch.

16. References

  1. PubMed: Adaptogenic and anxiolytic effects of Ashwagandha (PMID 23439798)

  2. NIH: Withania somnifera overview

  3. FDA GRAS Notice – Ashwagandha Extract

17. Contact & Verification

📧 Email: sales@aiherba.com | info@aiherba.com | liaodaohai@gmail.com
🌐 Website: www.aiherba.com
🏭 Manufacturer: Shaanxi Zhonghong Investment Technology Co., Ltd.
📍 Address: Xi’an, Shaanxi, China
🧾 Brand: AIHerba® | GMP Natural Extract Factory

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