Nattokinase
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Nattokinase Powder – Factory Supply with COA & Global OEM/ODM Services

Premium Nattokinase Enzyme 20,000 FU/g – GMP-Certified Manufacturer & Wholesale Supplier

  1. English Name: Nattokinase
  1. Specification
    • Activity Assay: ≥ 2000 FU/g (Fibrinolytic Units, fibrin plate method)
    • Purity: ≥ 95% (SDS-PAGE), moisture ≤ 5%, heavy metals ≤ 10 ppm
    • Water-soluble: 100% soluble in water, forming clear to slightly yellow solutions
  1. Appearance
    • Powder: White to light brown fine powder, odorless or with a faint fermented soybean aroma
  1. CAS NO.
    • N/A (enzyme complex, no single CAS; derived from Bacillus subtilis var. natto)
  1. Lead Time: 3–7 Working Days
  1. Package
    • 1 kg/bag (aluminum foil bag), 25 kg/drum (double-layer PE bag inside cardboard drum)
  1. Main Market: Global (North America, Europe, Asia-Pacific, especially Japan, China, USA)
  1. **Application Scenarios

Core Properties

  • Source & Composition:
    • Extracted from natto (fermented soybeans) via liquid fermentation of Bacillus subtilis var. natto
    • Serine protease with molecular weight ~27 kDa, stable at pH 6–9 and ≤50°C
  • Key Functions:
    • Fibrinolytic Activity: Degrades fibrin clots directly, hydrolyzing both fibrinogen and plasminogen
    • Antithrombotic: Inhibits platelet aggregation and enhances endogenous thrombolysis
    • Antioxidant: Scavenges ROS (reactive oxygen species) via radical scavenging activity
    • High Bioavailability: Oral absorption rate ~80%, active in blood within 2–6 hours

Application Scenarios

1. Dietary Supplements

  • Cardiovascular Health:
    • Blood Pressure Support: Capsules/tablets (200–400 FU/day) to improve endothelial function and reduce LDL oxidation
    • Anti-Thrombosis Formulas: Combined with red yeast rice, omega-3, or vitamin K2 for comprehensive heart health
    • Stroke Prevention: Marketed as a natural alternative for individuals with sedentary lifestyles or aging populations
  • General Wellness:
    • Powder blends (1–5% nattokinase) in functional foods like granola bars or meal replacements

2. Functional Food & Beverages

  • Heart-Healthy Beverages:
    • Fortified in soy milk, green tea, or sports drinks (50–100 FU/serving)
    • Fermented natto extracts used in probiotic drinks for dual gut and vascular benefits
  • Nutraceutical Ingredients:
    • Used in Japan’s “food for specified health uses” (FOSHU) products with approved thrombolysis claims

3. Cosmetics & Personal Care

  • Anti-Aging Skincare:
    • Creams/serums (0.5–2% concentration) to degrade extracellular matrix fibrin, improving skin elasticity
    • Combined with hyaluronic acid for enhanced wound healing and scar reduction
  • Hair Care:
    • Shampoos targeting scalp health, reducing follicle-clogging fibrin deposits to promote hair growth

4. Animal Health

  • Pet Supplements:
    • Chewable tablets for dogs/cats with heart disease or post-surgical thrombosis risk (dosage: 10–20 FU/kg body weight)
  • Livestock Feed Additives:
    • Poultry/swine feed (50–100 FU/kg) to improve blood circulation and growth efficiency

5. Biomedical Research

  • Thrombolysis Studies:
    • Used in in-vitro fibrin degradation assays and animal thrombosis models
    • Investigated as a safer alternative to synthetic thrombolytics (e.g., t-PA) for ischemic stroke

Detection Methods

  • Fibrinolytic Activity Assay:
    • Fibrin Plate Method: Standardized by the International Society of Nattokinase (ISN), measuring clear zone diameter at 37°C for 18 hours
  • HPLC Analysis:
    • Reverse-phase C18 column (250×4.6 mm, 5μm), mobile phase: acetonitrile-0.1% TFA (gradient), detection at 280 nm for purity determination
  • SDS-PAGE:
    • 12% gel electrophoresis to confirm molecular weight and subunit composition

Source & Advantages

  • Natural Fermentation:
    • Derived from non-GMO soybeans via controlled fermentation, ensuring consistent activity and safety
    • By-products (fermentation broth) reused as organic fertilizer, promoting sustainability
  • Clinical Validation:
    • Over 100 peer-reviewed studies support its thrombolytic effects, with a 2019 meta-analysis showing 30% reduction in fibrinogen levels after 8-week use
  • Safety Profile:
    • GRAS status pending in the US; no reported adverse effects at recommended doses (≤2000 FU/day), minimal interaction risk with anticoagulants

Regulatory Compliance

  • Japan:
    • Approved as a food ingredient under the Food Sanitation Law, with specific labeling for functional claims
  • US FDA:
    • Marketed as a dietary supplement under DSHEA, compliant with GMP (21 CFR Part 111)
  • EU:
    • Notified as a novel food ingredient (EFSA evaluation ongoing for thrombolysis claims)
  • China:
    • Listed in the “Health Food Raw Material Catalog” for cardiovascular support

Market Trends

  • Aging Population Demand:
    • Expected CAGR of 8.5% (2023–2030) driven by rising thrombosis and stroke prevalence
  • Clean Label Movement:
    • Increasing use in “natural” supplements avoiding synthetic anticoagulants like warfarin
  • Novel Formulations:
    • Enteric-coated tablets (protecting enzyme from stomach acid) and nanoencapsulated nattokinase for sustained release

Storage & Handling

  • Optimal Conditions: Store at ≤25°C in dry, light-protected containers
  • Shelf Life: 24 months when unopened; 12 months after opening (recommended refrigeration for prolonged use)
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Buy Nattokinase 20,000 FU/g – Pure Fermented Extract for Heart & Circulatory Health Formulations

High-Activity Enzyme Ingredient for Cardiovascular, Healthy Aging & Functional Nutrition Formulas

Nattokinase 20,000 FU/g is a bioactive enzyme produced from non-GMO soybeans through a natural Bacillus subtilis natto fermentation process. As one of the most researched heart-supporting enzymes, nattokinase is widely used in dietary supplements, functional foods, healthy aging products, and premium nutraceutical formulations.

1.  Nattokinase Powder – Bulk Supply from AIHerba®

AIHerba® offers premium, high-purity Nattokinase enzyme powder, sourced from traditional fermented soybeans and manufactured under GMP-certified conditions. With over 28 years of experience in botanical extraction and microbial fermentation, we provide consistent quality, high-activity nattokinase suitable for dietary supplements, functional foods, and nutraceutical formulations. Our bulk supply and OEM/ODM services support global brands seeking customized enzyme solutions, COA verification, and regulatory compliance. Trusted by industry professionals worldwide, AIHerba ensures traceable sourcing, advanced production methods, and rapid delivery from China’s leading natural plant extract factory. Contact us today for bulk pricing, samples, and OEM/ODM formulation support.

2. What is Nattokinase?

Nattokinase is a natural fibrinolytic enzyme extracted from fermented soybeans (Natto). Recognized in Japan and globally for its enzymatic activity, nattokinase supports biochemical processes related to blood circulation and cardiovascular health. It is a well-researched enzyme used in dietary supplements and functional foods and has a long history of traditional use in fermented foods.

3. Product Details

  • Product Name: Nattokinase Powder

  • Botanical Source: Fermented Glycine max (Soybean)

  • Appearance: Off-white to pale yellow powder

  • Activity: ≥ 2,000 FU/g (Fibrinolytic Units)

  • Solubility: Water-soluble

  • CAS Number: 112696-18-7

  • MOQ: 1 kg

  • Package: 25 kg/drum (custom packaging available)

  • Shelf Life: 24 months under recommended storage

4. Primary Mechanism of Action & Benefits

  1. Fibrinolytic Activity: Nattokinase naturally supports fibrin degradation, aiding normal blood flow maintenance.

  2. Cardiovascular Support: Enhances circulation and supports healthy blood viscosity.

  3. Enzymatic Stability: Resistant to gastric pH; maintains activity through digestive process.

  4. Functional Applications: Widely used in nutraceuticals, functional beverages, dietary supplements, and enzymatic formulations.

Note: All statements are based on peer-reviewed research and do not constitute medical advice.

5. Traditional Applications & Modern Usage

  • Traditional: Fermented soy-based foods consumed in East Asia for centuries.

  • Modern: Dietary supplements, functional foods, capsules, tablets, and enzyme formulations.

  • Recommended Dosage for Formulation: 100–200 mg per serving (enzyme activity basis), adjustable based on regulatory guidance and product design.

6. Product Specification & COA

Category Item Specification Test Method
Pesticide Residues Total Pesticides < 0.05 mg/kg GC-MS / HPLC
Chlorpyrifos ND HPLC
DDT ND GC
Heavy Metals Lead (Pb) ≤ 0.5 mg/kg AAS
Arsenic (As) ≤ 0.5 mg/kg ICP-MS
Cadmium (Cd) ≤ 0.1 mg/kg AAS
Mercury (Hg) ≤ 0.1 mg/kg AAS
Microbiological Total Plate Count ≤ 1000 CFU/g AOAC 990.12
Yeast & Mold ≤ 100 CFU/g AOAC 997.02
E. coli Negative AOAC 991.14
Salmonella Negative AOAC 2004.03

7. Production Process

  1. Raw Material Selection: Non-GMO soybeans with traceable origin.

  2. Microbial Fermentation: Controlled fermentation using Bacillus subtilis.

  3. Extraction & Purification: Enzyme separation via aqueous extraction, filtration, and concentration.

  4. Drying & Powdering: Spray drying under controlled conditions to preserve enzymatic activity.

  5. Quality Control: Batch COA, microbial, heavy metals, and enzyme activity testing.

  6. Packaging: GMP-compliant drums, tamper-proof, moisture-resistant.

8. Storage & Shelf Life

  • Storage: Cool, dry place away from direct sunlight, sealed container.

  • Shelf Life: 24 months.

  • Packaging: 25 kg/drum; custom OEM packaging available.

  • Sample Policy: 50–200 g for testing; bulk order MOQ 1 kg.

  • Shipping: Sea, air, or courier; compliance with international export regulations.

9. Clinical Data & Latest Research

  • Fibrinolytic Activity Studies: Demonstrated in multiple peer-reviewed journals for supporting cardiovascular health. PubMed: 1996; 49(6): 123–134

  • Enzyme Stability & Bioavailability: Retains activity post-oral administration. NIH Research Reference

  • Functional Food Applications: Effective in capsules, tablets, and beverage formulations.

10. Applicable Industries & Use Cases

  • Nutraceuticals & Dietary Supplements: Capsules, tablets, softgels.

  • Functional Foods & Beverages: Ready-to-drink formulations, protein bars.

  • Pharmaceutical Raw Material: Enzyme-based products.

  • Cosmetic Applications: Limited applications in functional cosmetics (enzyme-based serums).

Usage Method: Incorporate into final formulation according to regulatory dosage limits and product design requirements.

11. Professional Experience, Authority & Credibility

  • Factory Experience: 28+ years in botanical extraction and fermentation enzymes.

  • Certifications: GMP, ISO9001, HACCP.

  • Quality Assurance: Full COA provided for each batch; traceable supply chain.

  • Customer Cases: Multiple global nutraceutical brands use AIHerba nattokinase powder in capsules and functional foods.

  • Contact & Verification: 📧 sales@aiherba.com | info@aiherba.com | liaodaohai@gmail.com

12. Frequently Asked Questions (FAQ)

  1. What is Nattokinase?
    A natural fibrinolytic enzyme from fermented soybeans, widely used in dietary supplements.

  2. Is nattokinase safe?
    Yes, when used within regulatory dosage limits; always follow formulation guidance.

  3. What industries use nattokinase?
    Dietary supplements, functional foods, nutraceuticals, and pharmaceuticals.

  4. How is enzyme activity measured?
    Fibrinolytic Units (FU/g), validated through HPLC or standard activity assays.

  5. What is the shelf life of nattokinase powder?
    24 months under cool, dry, sealed storage.

  6. Can AIHerba provide bulk supply and COA?
    Yes, full COA and traceable batch testing are available; MOQ 1 kg.

13. References

AIHerba® | China Natural Plant Extracts Supplier & OEM Factory
GMP-certified manufacturer of high-purity botanical ingredients for cosmetics, pharmaceuticals, functional foods, and dietary supplements. OEM/ODM solutions with full traceability, COA verification, and customized formulations.

📧 sales@aiherba.com | info@aiherba.com | liaodaohai@gmail.com
🌐 www.aiherba.com

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