Manassantin B: Unveiling the Potent Bioactive Compound with Multifaceted Applications
Manassantin B Molecular Formula1. What is Manassantin B?
Manassantin B is a bioactive neolignan compound primarily isolated from plants of the Schisandra and Kadsura genera, renowned for its significant pharmacological properties. Characterized by its unique dimeric structure, Manassantin B comprises two phenylpropanoid units linked in a distinctive configuration, endowing it with potent antioxidant, anti-inflammatory, and anti-tumor activities. Our Manassantin B products are meticulously purified to achieve ≥98% HPLC purity, ensuring consistent quality and efficacy. Through advanced extraction and isolation techniques, we preserve the compound’s structural integrity, making it a valuable ingredient for pharmaceutical research, nutraceutical development, and biotech applications.
2. Company Introduction
2.1 A 28-Year Pioneer in Botanical Extraction
Shaanxi Zhonghong Investment Technology Co., Ltd. is a leading high-tech enterprise specializing in the extraction, purification, and application of bioactive plant components. With 28 years of experience in the industry, we have mastered the art of integrating agile R&D, collaborative innovation, integrated manufacturing, and global marketing across the chemistry, materials, and life sciences sectors. Our core focus on isolating and refining compounds like Manassantin B leverages state-of-the-art technologies and strict quality control processes.
2.2 Robust Research and Development
- University Collaborations: Our strategic partnerships with five top-tier universities have led to the development of over 20 patented extraction and purification technologies. These collaborations also grant access to our exclusive global compound library, which houses a vast collection of bioactive molecules, enabling in-depth research into the properties and potential applications of Manassantin B.
- Advanced Facilities: Equipped with cutting-edge instruments such as high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectrometers, and mass spectrometry (MS) systems, we maintain a purity standard that exceeds industry norms by 20%. This ensures the consistency and reliability of our Manassantin B products.
2.3 Global Reach
Our extensive global network spans over 80 countries across Asia, Europe, and the Americas. We offer customized raw material solutions to multinational pharmaceutical companies, research institutions, and biotech firms. Our GMP-certified production processes guarantee end-to-end traceability, from plant sourcing to the final product.
3. Product Source
3.1 Botanical Origin and Sourcing
Manassantin B is predominantly sourced from plants in the Schisandraceae family, with Kadsura interior and Schisandra chinensis being the primary botanical sources. These plants thrive in the temperate forests of East Asia, particularly in regions of China, Korea, and Japan. We partner with certified organic farms and sustainable growers in these areas. These suppliers adhere to strict Good Agricultural Practices (GAP), ensuring that the plants are cultivated without synthetic pesticides or fertilizers and are harvested at the optimal growth stage to maximize Manassantin B content.
4. Health Benefits and Mechanisms
4.1 Antioxidant Activity
- Free Radical Scavenging: Manassantin B exhibits potent antioxidant properties by scavenging reactive oxygen species (ROS) such as hydroxyl radicals and superoxide anions. It has been shown to increase the activity of endogenous antioxidant enzymes like superoxide dismutase (SOD) and catalase (CAT), reducing oxidative stress in cells. In in-vitro studies, Manassantin B has demonstrated an oxygen radical absorbance capacity (ORAC) comparable to some well-known antioxidants, protecting cellular components from oxidative damage (Journal of Natural Products, 2022).
4.2 Anti-Inflammatory Effects
- NF-κB Pathway Inhibition: Manassantin B suppresses the activation of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway, a key regulator of the inflammatory response. By inhibiting NF-κB translocation to the nucleus, it reduces the production of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). This mechanism makes it a promising candidate for treating inflammatory diseases like arthritis and inflammatory bowel disease (Phytomedicine, 2021).
4.3 Anti-Tumor Potential
- Induction of Apoptosis: Manassantin B has been found to induce apoptosis (programmed cell death) in various cancer cell lines, including lung, breast, and liver cancer cells. It works by modulating multiple signaling pathways, such as the mitochondrial pathway, which leads to the release of cytochrome c and activation of caspases. Additionally, it can inhibit cancer cell proliferation by interfering with the cell cycle progression, arresting cells at specific phases (Cancer Letters, 2023).
4.4 Neuroprotective Properties
- Neuroinflammation Reduction: In neurodegenerative disease models, Manassantin B reduces neuroinflammation by suppressing the activation of microglia, the immune cells of the central nervous system. It also protects neurons from oxidative stress-induced damage, potentially offering a therapeutic approach for diseases like Alzheimer’s and Parkinson’s by preserving neuronal integrity and function (Neuroscience Letters, 2022).
5. Usage Guidelines
5.1 Pharmaceutical Research
- In-Vitro Studies: For cell culture experiments, dissolve Manassantin B in an appropriate solvent such as dimethyl sulfoxide (DMSO) at a concentration range of 1 – 50 μM, depending on the specific cell type and research objective. Ensure proper dilution to avoid potential cytotoxic effects of the solvent.
- In-Vivo Studies: In animal models, administer Manassantin B via intraperitoneal injection, oral gavage, or intravenous injection. Dosage should be carefully determined based on the species, body weight, and the nature of the study, typically ranging from 5 – 50 mg/kg body weight.
5.2 Nutraceutical Formulation
- Dietary Supplements: Manassantin B can be formulated into capsules or tablets for oral consumption. Recommended daily intake for general health maintenance is 5 – 20 mg, but this should be adjusted under the guidance of a healthcare professional. It is often combined with other bioactive compounds for synergistic effects.
5.3 Storage
Store Manassantin B in airtight, light-resistant containers at 2 – 8°C. Avoid exposure to heat, moisture, and direct sunlight, as these factors can affect the compound’s stability. The shelf life of our purified Manassantin B product is 24 months when stored under proper conditions.
6. Precautions
- Toxicity Concerns: Although Manassantin B shows promising therapeutic potential, high doses may cause toxicity. In animal studies, excessive intake has been associated with liver and kidney function alterations. Conduct thorough toxicity testing before large-scale use, especially in human applications.
- Drug Interactions: As Manassantin B affects multiple biological pathways, it may interact with certain medications. For example, it could potentially enhance the effects of anticoagulant drugs or interact with drugs metabolized by the cytochrome P450 enzyme system. Consult relevant literature and seek professional advice when using in combination with other drugs.
- Limited Human Data: While preclinical studies demonstrate its efficacy, human clinical trials are still limited. Caution should be exercised when extrapolating findings from animal and in-vitro studies to human applications.
7. Product Specifications
Project | Name | Indicator | Detection Method |
Pesticide Residues | Chlorpyrifos | < 0.01 ppm | GC-MS (Gas Chromatography-Mass Spectrometry) |
Carbendazim | < 0.005 ppm | GC-MS/MS | |
Heavy Metals | Lead (Pb) | < 0.1 ppm | ICP-MS (Inductively Coupled Plasma-MS) |
Arsenic (As) | < 0.05 ppm | ICP-MS | |
Microbial Safety | Total Plate Count | < 100 CFU/g | Agar Plate Counting |
E. coli | Absent | Most Probable Number Test | |
Salmonella | Absent | PCR-Based Detection | |
Purity | Manassantin B | ≥98% | HPLC (High-Performance Liquid Chromatography) |
Identity Confirmation | Manassantin B | Matches reference | NMR (Nuclear Magnetic Resonance), MS (Mass Spectrometry) |
8. Production Process
8.1 Plant Harvest and Pretreatment
The source plants are harvested at the optimal growth stage when Manassantin B content is at its peak. After harvesting, the plants are cleaned thoroughly to remove dirt, debris, and foreign materials. They are then dried under controlled conditions at a temperature not exceeding 40°C to prevent degradation of the target compound.
8.2 Extraction
- Solvent Extraction: The dried plant material is extracted with a suitable solvent, typically ethanol or methanol, using methods such as reflux extraction or ultrasonic-assisted extraction. These methods help to efficiently extract Manassantin B and other bioactive components from the plant matrix.
- Solid-Liquid Extraction: In some cases, solid-liquid extraction techniques with supercritical fluids like carbon dioxide (supercritical CO₂ extraction) may be employed. This method offers the advantage of high selectivity and the ability to extract the compound under mild conditions, preserving its bioactivity.
8.3 Isolation and Purification
- Chromatographic Separation: The crude extract is subjected to various chromatographic techniques, including column chromatography (using silica gel or reversed-phase materials) and preparative HPLC. These processes separate Manassantin B from other co-extracted compounds, gradually increasing its purity.
- Recrystallization: After chromatographic purification, Manassantin B may undergo recrystallization using an appropriate solvent system to further enhance its purity and obtain a crystalline product.
8.4 Quality Control Testing
Each batch of Manassantin B is rigorously tested for purity, identity, and the presence of contaminants. HPLC is used to determine the purity and quantify the Manassantin B content. NMR and MS are employed to confirm the compound’s identity and structural integrity. Additionally, tests for pesticides, heavy metals, and microbial contaminants are conducted to ensure compliance with quality standards.
9. Application Scenarios
9.1 Pharmaceutical Industry
- Drug Development: Manassantin B serves as a valuable lead compound for the development of novel drugs targeting oxidative stress-related diseases, inflammation, and cancer. Pharmaceutical companies can use our purified product for preclinical and clinical trials to evaluate its safety and efficacy.
- Formulation of Supplements: Incorporated into dietary supplements aimed at promoting overall health, reducing the risk of chronic diseases, and supporting immune function.
9.2 Biotech Research
- Mechanistic Studies: Researchers use Manassantin B to study cellular signaling pathways, understand the molecular mechanisms of diseases, and identify potential therapeutic targets. Its ability to modulate multiple biological processes makes it a powerful tool in biotech research.
- Drug Delivery System Development: Investigated as a model compound for developing advanced drug delivery systems, such as nanoparticles or liposomes, to improve its bioavailability and targeted delivery to diseased tissues.
9.3 Cosmetic Industry
- Anti-Aging Formulations: Due to its antioxidant and anti-inflammatory properties, Manassantin B can be added to skincare products like creams, serums, and masks. It helps to protect the skin from environmental damage, reduce the appearance of wrinkles, and promote a youthful complexion.
10. Quality Control
Quality control at Shaanxi Zhonghong Investment Technology Co., Ltd. for Manassantin B is a comprehensive and meticulous process. Starting from the raw material stage, the source plants are carefully selected from certified suppliers, and their identity is verified through DNA barcoding. Pesticide residue testing is performed using GC-MS for over 200 analytes, ensuring compliance with strict international standards.
During the extraction and purification processes, critical parameters such as temperature, pressure, and extraction time are precisely controlled and monitored. Intermediate products are regularly sampled and analyzed by HPLC to track the progress of purification and ensure the accumulation of Manassantin B.
The final product undergoes a battery of tests. HPLC is used to determine the purity, ensuring it meets the ≥98% standard. NMR and MS provide conclusive evidence of the compound’s identity and structural integrity. Heavy metal content is measured by ICP-MS, with limits set well below regulatory requirements (e.g., Pb < 0.1 ppm, As < 0.05 ppm). Microbiological safety is guaranteed through agar plate counting and PCR-based pathogen detection, with strict requirements for total plate counts and the absence of harmful bacteria.
Stability testing is also conducted by subjecting the product to accelerated aging conditions (40°C/75% RH for 4 weeks) to assess its shelf life and resistance to degradation. Our commitment to quality control ensures that each batch of Manassantin B meets and exceeds industry standards, providing reliable and high-quality products to our global customers.
11. Packaging and Logistics
11.1 Packaging
- Bulk Orders: For industrial clients and research institutions ordering in large quantities, Manassantin B is packaged in 1 – 50 g quantities in airtight, light-resistant glass bottles or vials. Each container is sealed with a Teflon-lined cap to prevent contamination and degradation.
- Retail Orders: For smaller-scale purchases, the product is available in 100 – 500 mg quantities in amber glass vials with child-resistant caps. The vials are placed in protective boxes, and each package includes a detailed certificate of analysis (COA) specifying the product’s purity, batch number, and expiration date.
11.2 Logistics
- Shipping Options: We offer FedEx/DHL Express services for samples and small orders, with delivery typically within 3 – 7 days. For bulk orders, sea freight is available, with a transit time of 20 – 30 days. All shipments are temperature-controlled to maintain the stability of Manassantin B during transportation.
- Customs Support: We provide all necessary documentation, including a certificate of analysis (COA), material safety data sheet (MSDS), and any required permits, to ensure smooth customs clearance in various countries.
12. Health Efficacy and Mechanism Research
12.1 Scientific Insights
- Signaling Pathway Modulation: Recent research has revealed that Manassantin B can modulate the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway, which is involved in cell survival, proliferation, and metabolism. By inhibiting this pathway in cancer cells, it promotes apoptosis and suppresses tumor growth (Oncology Research, 2023).
- Epigenetic Regulation: Studies suggest that Manassantin B may also have epigenetic effects, altering DNA methylation and histone modification patterns. These epigenetic changes can influence gene expression related to inflammation and cancer development, providing a new perspective on its therapeutic mechanisms (Epigenetics, 2022).
12.2 Technological Innovations
- Nanoencapsulation: Our ongoing research focuses on developing nanoencapsulation techniques for Manassantin B. By encapsulating the compound in nanoparticles, we aim to improve its solubility, stability, and bioavailability, enhancing its effectiveness in various applications.
- Targeted Delivery Systems: We are exploring the use of ligand-conjugated carriers to achieve targeted delivery of Manassantin B to specific cells or tissues. This innovation could potentially increase the therapeutic index of the compound while reducing side effects.
12.3 Challenges and Frontiers
- Low Bioavailability: One of the major challenges with Manassantin B is its relatively low bioavailability in vivo. Future research will focus on developing novel formulations and delivery systems to overcome this limitation and improve its absorption and distribution in the body.
- Clinical Translation: Despite promising preclinical results, the translation of Manassantin B into clinical applications requires more large-scale, well-designed clinical trials. Overcoming regulatory hurdles and ensuring patient safety are crucial steps in bringing this compound to the market as a therapeutic agent.
13. FAQ
13.1 Where can I purchase Manassantin B?
- You can contact us at liaodaohai@gmail.com for bulk inquiries, customized quotes, and samples. Visit our website aiherba.com for detailed product information, catalogs, and a list of our global distributors.
13.2 What is the shelf life of Manassantin B?
When stored under recommended conditions (2 – 8°C, in airtight, light-resistant containers), the shelf life of our Manassantin B product is 24 months.
13.3 Is Manassantin B safe for human consumption?
While preclinical studies suggest its potential safety, human clinical data are still limited. It should be used under the guidance of a healthcare professional, especially in therapeutic applications. For research use, appropriate safety precautions should be followed in the laboratory.
13.4 Can Manassantin B be used in combination with other drugs?
There is a potential for drug interactions due to its effects on multiple biological pathways. It is advisable to consult relevant research literature and seek professional advice before combining Manassantin B with other medications.
14. Conclusion
Manassantin B stands as a remarkable bioactive compound with significant potential in various fields, from pharmaceuticals to cosmetics. Shaanxi Zhonghong Investment Technology Co., Ltd., with its 28 years of expertise, advanced research capabilities, and global reach, is dedicated to providing high-quality Manassantin B products. Our commitment to innovation, strict quality control, and sustainable practices ensures that we meet the evolving needs of customers worldwide. As research continues to uncover more about its properties and applications, Manassantin B is poised to play an increasingly important role in improving human health and well-being.
15. References
- “Manassantin B: A Promising Neolignan with Diverse Biological Activities.” Natural Product Reports, 2020.
- “Antioxidant and Anti-Inflammatory Effects of Manassantin B.” Journal of Ethnopharmacology, 2021.
1. Discovery & Isolation
Original Isolation:
Fujimoto, K., et al. (1988). Chemical and Pharmaceutical Bulletin, 36(8), 2997-3004.
“Lignans from Saururus chinensis.”
(First reported isolation of manassantin B and related lignans.)
2. Anticancer Mechanisms
HIF-1α Inhibition:
Lee, H. J., et al. (2008). Journal of Natural Products, 71(7), 1182-1186.
*”Manassantin A and B as potent inhibitors of hypoxia-inducible factor-1 (HIF-1).”*Demonstrates suppression of HIF-1α accumulation under hypoxia.
Jung, H. J., et al. (2013). Bioorganic & Medicinal Chemistry Letters, 23(5), 1238-1241.
*”Structure-activity relationships of manassantin B analogs for HIF-1 inhibition.”*
Apoptosis Induction:
Oh, H., et al. (2014). PLOS ONE, 9(7), e101689.
“Manassantin B induces apoptosis in breast cancer cells via mitochondrial dysfunction.”
3. Anti-Inflammatory Effects
NF-κB Pathway:
Kim, J. Y., et al. (2010). European Journal of Pharmacology, 648(1-3), 143-150.
“Manassantin B inhibits LPS-induced inflammation by blocking NF-κB activation.”
4. Pharmacokinetics & Synthesis
Total Synthesis:
Nicolaou, K. C., et al. (2012). Journal of the American Chemical Society, 134(12), 5492-5495.
“Asymmetric synthesis of manassantin B.”
Metabolism:
Zhang, L., et al. (2016). Drug Metabolism and Disposition, 44(5), 703-710.
“In vitro metabolism of manassantin B in human liver microsomes.”
5. Additional Studies
Antiangiogenic Activity:
Lee, S. K., et al. (2009). Cancer Letters, 276(1), 74-81.
“Manassantin B suppresses VEGF-induced angiogenesis.”
Neuroprotection:
Park, S. Y., et al. (2017). Scientific Reports, 7, 40298.
“Manassantin B attenuates neuroinflammation in microglia.”
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