Camptothecin: Nature’s Potent Topoisomerase Inhibitor for Oncology & Analysis | Supplier & Manufacturer

1. What is Camptothecin?

Camptothecin (CPT) is a pentacyclic quinoline alkaloid initially remoted from the bark/stems of the Chinese language Comfortable Tree (Camptotheca acuminata). It features as a potent topoisomerase I (Topo I) inhibitor, binding to the Topo I-DNA advanced and stopping DNA religation throughout replication. This triggers replication fork collapse, double-strand DNA breaks, আৰু S-phase selective apoptosis in quickly dividing cells. Its distinctive mechanism underpins its significance in oncology therapeutics আৰু most cancers biology analysis.

২/ যোগান, ৰাসায়নিক ধৰ্ম আৰু চিনাক্তকৰ্তা

• যোগান: Primarily extracted from Camptotheca acuminata bark/seeds. Semi-synthesis from plant precursors is widespread.

• ৰাসায়নিক ধৰ্ম:

  • ৰাসায়নিক নাম: (S)-4-Ethyl-4-hydroxy-1H-pyrano[3′,4′:6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione

  • আণৱিক উপাদানসমূহ: C₂₀H₁₆N₂O₄

  • আণৱিক ওজন: 348.36 g/mol

  • ৰূপ: Pale yellow to gentle inexperienced crystalline powder

  • দ্ৰৱণীয়তা: Lactone ring pH-dependence – Soluble in DMSO, DMF, chloroform (closed lactone kind, energetic); hydrolyzes to inactive carboxylate kind in aqueous pH >7.

  • স্থিৰতা: Mild and pH-sensitive. Requires strict management of lactone ring integrity for bioactivity.

• মূল চিনাক্তকৰ্তাসমূহ:

  • CAS: 7689-03-4

  • ইচি পৰিমাণ (EINECS): 231-684-4

  • এম এফ: C₂₀H₁₆N₂O₄

  • মেগাৱাট: 348.36

3. Purity, Efficacy, Security & Dosage

• অনুকূল পণ্য: The “finest” camptothecin options >99% HPLC purity, >95% lactone kind content material (important for exercise), and undetectable contaminants. Shaanxi Zhonghong achieves this by way of proprietary pH-controlled crystallization আৰু লাইঅ’ফিলাইজেচন.

• Well being Benefits & Functions:

  • Anticancer Exercise: Core mechanism: Topo I inhibition → DNA harm → S-phase apoptosis. Foundation for FDA-approved derivatives (Irinotecan, Topotecan) treating colorectal, ovarian, SCLC, cervical cancers.

  • Antiviral/Antiparasitic Analysis: Rising exercise towards HIV-1, HSV, Leishmania in preclinical fashions.

  • (Be aware: Nattokinase primarily exhibits anticoagulant/thrombolytic results; direct kidney advantages are much less established vs. potential CPT nephrotoxicity issues).

• Dosage & Administration:

  • NOT a dietary complement. Used clinically as IV derivatives below strict medical supervision.

  • Analysis doses differ (usually 0.1-100 µM ইন ভিট্ৰ’ত; mg/kg ranges ইন ভিভ'ত). No secure each day consumption for self-administration exists.

• Important Precautions & Aspect Results:

  • Extreme Toxicity: Myelosuppression (neutropenia, thrombocytopenia), extreme diarrhea (cholinergic & delayed), hemorrhagic cystitis, hepatotoxicity.

  • Lactone Instability: Speedy hydrolysis to inactive carboxylate at physiological pH reduces efficacy.

  • বিৰোধীতা: Being pregnant/lactation (teratogenic), pre-existing bone marrow melancholy, energetic infections.

  • ৰ সৈতে মোকাবিলা কৰা: Cytotoxic – Requires PPE (gloves, masks, lab coat). Keep away from inhalation/pores and skin contact.

4. Shaanxi Zhonghong: Your Premier Camptothecin Supply

Shaanxi Zhonghong Investment Technology Co., Ltd. is a cGMP/ISO-certified chief in high-purity plant-derived APIs. With 28+ years in bioactive phytochemistry, we focus on:

• Core Capabilities:

  • বৈজ্ঞানিক উৎকৃষ্টতা: 5 College Joint Labs, 20+ Patents (incl. CPT stabilization tech), Unique Natural Compound Library.

  • Slicing-Edge Analytics: HPLC-PDA/ELSD (purity), ইউপিএলচি-কিউটিঅ’এফ-এমএছ (impurity profiling), Superconducting FT-NMR (construction ID), আইচিপি-এমএছ (গধুৰ ধাতু), জিচি-এমএছ/এমএছ (residual solvents). Purity >99%, exceeding USP by 20%.

  • আন্তৰ্জাতিক অৰ্জন কৰক: Provides ৮০+ জাতি – Pharma, Biotech, Tutorial Analysis.

  • উলম্ব সংহতি: বহনক্ষম উৎস → GMP Extraction → pH-Managed Purification → লাইঅ’ফিলাইজেচন.

5. Rigorous Product Specs

৬/ উচ্চমানৰ উৎপাদন কাৰ্য্যপ্ৰবাহ

1. Sustainable Harvesting: Camptotheca acuminata cultivated below GACP tips.

2. নিৰ্বাচিত নিষ্কাশন: Ethanol/Water অথবা Supercritical CO₂ extraction of bark/seeds.

3. মুখ্য বিশুদ্ধকৰণ: Liquid-liquid partitioning (CH₂Cl₂/H₂O), adsorbent chromatography (SiO₂, polyamide).

4. Lactone Stabilization: pH-controlled crystallization (pH 3.5-4.5) to maximise lactone yield.

5. Last Purification: প্ৰস্তুতিমূলক এইচ পি এল চি (C18 column, MeOH/H₂O/TFA cell section).

6. শুকুৱাই লোৱা: লাইঅ’ফিলাইজেচন (-50°C, <10 Pa) to protect lactone ring & stability.

7. QC লঞ্চ: সম্পূৰ্ণ CoA verifying identification, purity, lactone content material, contaminants.

7. Key Application Areas

• Oncology Medicine: Precursor for Irinotecan (CPT-11), Topotecan, Belotecan.

• Most cancers Analysis: Software compound for DNA harm response, apoptosis, drug resistance research.

• Drug Supply Techniques: Loaded into liposomes, nanoparticles, polymer conjugates to boost solubility/concentrating on.

• Antiviral/Antiparasitic Improvement: Investigational agent for novel therapeutics.

8. cGMP উচ্চমানৰ ব্যৱস্থাপনা প্ৰটোকল
Shaanxi Zhonghong employs a tiered QC technique:

• চিনাক্তকৰণ: FT-NMR (¹H/¹³C), HPLC-UV/PDA spectral match vs. USP reference customary.

• Assay & Impurities: HPLC-ELSD/PDA quantifies CPT (≥99.0%) and detects ≤0.1% associated compounds (10-deacetylcamptothecin, camptothecin carboxylate).

• Lactone Content: Validated pH-specific RP-HPLC methodology distinguishes lactone/carboxylate types.

• দূষক পদাৰ্থ: আইচিপি-এমএছ for heavy metals (USP <232>), জিচি-এমএছ/এমএছ for 500+ pesticides (EU 2021/601), এল চি-এম এছ/এম এছ for mycotoxins, USP <61>/<62> for microbiology.

• স্থিৰতা: Actual-time & accelerated আইচিএইচ কিউ১এ(আৰ২) research verify 24-month shelf-life at -20°C (desiccated, argon ambiance).

9. Safe Packaging & Logistics

• মেজৰ পেক: Amber glass vials sealed below argon, full of desiccant.

• মাধ্যমিক পেক: Vacuum-sealed aluminum luggage ভিতৰত UN-certified HDPE containers.

• সংৰক্ষণ: -20°C ± 5°C; defend from gentle/moisture.

• বিলি কৰা: International cold-chain logistics (-15°C to -25°C) by way of DHL FedEx UltraCold.

10. Mechanisms, Functions & Challenges

• ব্যৱস্থা: Traps Topo I-DNA cleavable complexes → Collision with replication forks → DSBs → ATR/Chk1 activation → p53-mediated apoptosis.

• উদ্ভাৱন: ঝংহংৰ PEGylated liposomal CPT (Patent: CN202210XXXXXX) enhances tumor concentrating on.

• বিশ্লেষণ সীমান্ত:

  • Novel Analogues: 7-Silylcamptothecins (improved lactone stability).

  • মিশ্ৰণ চিকিৎসা: With PARP inhibitors, immunotherapy.

  • Biomarkers: Predictive biomarkers for CPT sensitivity (ERCC1, Topo I ranges).

• প্ৰত্যাহ্বানসমূহ: Lactone instability, myelosuppression, ABCG2-mediated drug resistance, tumor heterogeneity.

11. Steadily Requested Questions (FAQ)

• Q1: Can I take Camptothecin as a complement?

  • A: Completely NOT. CPT is a potent cytotoxic compound with extreme unintended effects. Use solely below medical supervision in FDA-approved by-product types (e.g., Irinotecan) or for managed analysis.

• Q2: Why is lactone kind important?

  • উঃ: Solely the closed lactone ring binds Topo I. The open carboxylate kind is inactive and will trigger renal toxicity. Zhonghong ensures ≥95% lactone content material by way of lyophilization.

• Q3: How does Camptothecin differ from Nattokinase for kidneys?

  • উঃ: CPT carries nephrotoxicity dangers (tubular harm). Nattokinase, a fibrinolytic enzyme, lacks strong proof for direct kidney advantages. Neither is nephroprotective.

• This autumn: What analytical strategies verify Zhonghong’s CPT purity?

  • উঃ: HPLC-PDA/ELSD (purity), ইউপিএলচি-কিউটিঅ’এফ-এমএছ (impurities), FT-NMR (construction), pH-HPLC (lactone quantification), আইচিপি-এমএছ (heavy metals).

• Q5: Do you supply Camptothecin derivatives?

  • উঃ: Sure. GMP-grade 10-Hydroxycamptothecin, SN-38 (Irinotecan metabolite), customized synthesis obtainable.

12. The place to Purchase?
Supply research-grade & GMP Camptothecin:

• ই-মেইল: liaodaohai@gmail.com ত যোগাযোগ কৰক

• ইণ্টাৰনেট: www.aiherba.com ত যোগদান কৰক

• অনুৰোধ: COA, MSDS, customized synthesis quotes.

১৩) উপসংহাৰ
Camptothecin stays a cornerstone of topoisomerase-targeted oncology. Its medical impression by way of semisynthetic derivatives underscores its irreplaceable function. Success hinges on securing ultra-pure, lactone-stabilized materials with verifiable traceability. Shaanxi Zhonghong delivers >99% HPLC purity CPT যোগেদি patented stabilization expertise, cGMP অনুসৰণ কৰা, আৰু unmatched analytical rigor – empowering drug discovery and superior therapeutics.

১৪) তথ্যসূত্ৰ

1. Wall ME, et al. (1966). জে আম কেম ছ'চ. 88:3888–3890. [DOI:10.1021/ja00968a057]

2. Hsiang YH, et al. (1985). J Biol Chem. 260:14873–14878. [PMID:2997227]

3. Pommier Y. (2006). Nat Rev Most cancers. 6:789–802. [DOI:10.1038/nrc1977]

4. USP-NF Monograph: Camptothecin. USP44-NF39.

5. ICH Q3C (R8): Residual Solvents. (2021).

6. FDA Steerage: Irinotecan Label. (2023).

7. Thomas CJ, et al. (2004). জে মেড কেম. 47:3972–3981. [DOI:10.1021/jm030660+]

8. Du W, et al. (2022). Acta Pharm Sin B. 12:3006–3025. [DOI:10.1016/j.apsb.2022.03.011]

9. GLS-010-IPHASE Research Protocol (NCT04501563).

10. EMA Evaluation Report: Topotecan (2020). EMA/CHMP/89262/2020.