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Camptothecin supplier bulk factory Wholesale buy Extract factory

  1. 영어 이름: Camptothecin
  1. 사양
    • 청정: Camptothecin ≥ 98% (HPLC); High – purity grade: ≥ 99% (HPLC)
    • 용해도: Sparingly soluble in water (approx. 0.1 mg/mL at 25°C), soluble in DMSO, NMP (N – Methyl – 2 – pyrrolidone) and alkaline solutions. Water – soluble derivatives (e.g., sodium salt) have improved solubility.
    • 녹는점: 260 – 264°C (decomposes)
    • 잔류 용매: DMSO ≤ 0.5%, NMP ≤ 0.5%
    • 중금속: ≤ 10ppm
    • 입자 크기: Standard powder ≤ 100 μm; micronized powder ≤ 5 μm for enhanced bioavailability in formulations
  1. 모습
    • Pale yellow to yellowish – green crystalline powder, odorless
  1. CAS 번호：7689 – 03 – 4
  1. 리드타임: 7~10일
  1. 패키지
    • 100 mg/vial (sterile glass vial), 1 g/aluminum foil – lined bag; stored under nitrogen to prevent oxidation
  1. 주요 시장: Global pharmaceutical and research markets, with significant demand in North America, Europe, and Asia, especially in oncology research and drug development
  1. 응용 프로그램 시나리오

핵심 속성

  • 분자식: C₂₀H₁₆N₂O₄
  • 화학 구조: A pentacyclic quinoline alkaloid, featuring a unique camptothecin ring system with a lactone moiety, which is crucial for its biological activity.
  • 주요 특징
    • Topoisomerase Inhibitor: Specifically targets DNA topoisomerase I, preventing the religation of DNA strands during replication, leading to DNA damage and cell death in rapidly dividing cells.
    • 항종양 활성: Potent cytotoxic effects against a wide range of cancer cell lines, including colorectal, lung, ovarian, and pancreatic cancers.
    • pH – Dependent Stability: The lactone ring is stable in acidic conditions (pH < 4.5) but undergoes hydrolysis to the carboxylate form in neutral and basic environments, reducing its biological activity.
    • 고효능: Low therapeutic doses are required due to its strong inhibitory effect on DNA replication, but this also necessitates careful handling to manage potential toxicity.

응용 프로그램 시나리오

1. Oncology Research

  • Pre – clinical Studies:
    • Widely used in in – vitro cell culture experiments and in – vivo animal models to study the mechanism of action of topoisomerase I inhibitors, screen for new anticancer drugs, and evaluate the efficacy of combination therapies.
  • 약물 개발:
    • Serves as a lead compound for the development of second – generation and third – generation camptothecin – based drugs. Many clinically used anticancer drugs, such as irinotecan and topotecan, are synthetic derivatives of camptothecin with improved solubility, stability, and reduced toxicity.

2. 제약 산업

  • Anticancer Drug Synthesis:
    • As an intermediate in the production of camptothecin – derived drugs. Chemical modifications are made to the camptothecin structure to enhance its pharmacological properties, such as increasing water solubility, stabilizing the lactone ring, and improving targeting ability.
  • 품질 관리:
    • Used as a reference standard for the analysis and quality control of camptothecin – based drugs, ensuring the consistency and purity of pharmaceutical products.

3. 생물의학 연구

  • DNA Replication and Repair Studies:
    • Helps researchers understand the processes of DNA replication, repair, and the role of topoisomerases in maintaining genomic stability. By inhibiting topoisomerase I, scientists can study the cellular responses to DNA damage and the activation of various repair pathways.
  • Drug Resistance Research:
    • Investigated to understand the mechanisms of drug resistance in cancer cells towards camptothecin and its derivatives. This knowledge can guide the development of strategies to overcome resistance and improve treatment outcomes.

탐지 방법

  • 고성능 액체 크로마토그래피(HPLC):
    • Column: C18 (250 × 4.6 mm, 5 μm), Mobile Phase: Acetonitrile – 0.1% formic acid (gradient elution), Flow Rate: 1.0 mL/min, Detection Wavelength: 254 nm for quantification.
  • Ultra – Performance Liquid Chromatography – Mass Spectrometry (UPLC – MS):
    • Used for the identification and quantification of camptothecin and its metabolites in biological samples. It provides high – sensitivity and high – resolution analysis, crucial for pharmacokinetic and pharmacodynamic studies.
  • 푸리에 변환 적외선 분광법(FTIR):
    • Helps in confirming the chemical structure of camptothecin by analyzing the characteristic absorption peaks of its functional groups, ensuring the integrity of the compound during production and storage.

출처 및 장점

  • 천연 자원: Originally isolated from the bark and wood of the Chinese tree Camptotheca acuminata. However, due to the low content of camptothecin in the plant (0.01 – 0.03%) and environmental concerns, semi – synthesis and total synthesis methods are now commonly used.
  • 치료적 잠재력: Its unique mechanism of action makes it a valuable starting point for the development of highly effective anticancer drugs, contributing significantly to the field of oncology.
  • 연구 유틸리티: A crucial tool in biomedical research, enabling a deeper understanding of fundamental biological processes related to DNA metabolism and cancer biology.

규정 준수

  • 미국 식품의약국: Regulated as an investigational new drug (IND) for research purposes and as a pharmaceutical ingredient for approved camptothecin – based drugs. Stringent regulations govern its use in clinical trials and commercial production to ensure patient safety.
  • EU 규정: Subject to strict regulations under the European Medicines Agency (EMA). Clinical trials involving camptothecin and its derivatives must comply with Good Clinical Practice (GCP) guidelines, and marketing authorization is required for commercial use.
  • 중국 국가광물자원관리국: Monitored closely in China, especially for drugs derived from camptothecin. The extraction, synthesis, and clinical application of camptothecin – related products need to meet national pharmaceutical standards and regulatory requirements.
범주: ,

Camptothecin: Nature’s Potent Topoisomerase Inhibitor for Oncology & Analysis | Supplier & Manufacturer

1. What is Camptothecin?

Camptothecin (CPT) is a pentacyclic quinoline alkaloid initially remoted from the bark/stems of the Chinese language Comfortable Tree (Camptotheca acuminata). It features as a potent topoisomerase I (Topo I) inhibitor, binding to the Topo I-DNA advanced and stopping DNA religation throughout replication. This triggers replication fork collapse, double-strand DNA breaks, 그리고 S-phase selective apoptosis in quickly dividing cells. Its distinctive mechanism underpins its significance in oncology therapeutics 그리고 most cancers biology analysis.

2. 공급, 화학적 특성 및 식별자

  • 공급: 주로 다음에서 추출됨 Camptotheca acuminata bark/seeds. Semi-synthesis from plant precursors is widespread.

  • 화학적 특성:

    • 화학명: (S)-4-Ethyl-4-hydroxy-1H-pyrano[3′,4′:6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione

    • 분자 구성 요소: C₂₀H₁₆N₂O₄

    • 분자량: 348.36 g/mol

    • 모습: Pale yellow to gentle inexperienced crystalline powder

    • 용해도: Lactone ring pH-dependence – Soluble in DMSO, DMF, chloroform (closed lactone kind, energetic); hydrolyzes to inactive carboxylate kind in aqueous pH >7.

    • 안정: Mild and pH-sensitive. Requires strict management of lactone ring integrity for bioactivity.

  • 주요 식별자:

    • CAS: 7689-03-4

    • EC 수량(EINECS): 231-684-4

    • MF: C₂₀H₁₆N₂O₄

    • MW: 348.36

3. Purity, Efficacy, Security & Dosage

  • 최적의 제품: The “finest” camptothecin options >99% HPLC purity, >95% lactone kind content material (important for exercise), and undetectable contaminants. Shaanxi Zhonghong achieves this by way of proprietary pH-controlled crystallization 그리고 동결건조.

  • Well being Benefits & Functions:

    • Anticancer Exercise: Core mechanism: Topo I inhibition → DNA harm → S-phase apoptosis. Foundation for FDA-approved derivatives (Irinotecan, Topotecan) treating colorectal, ovarian, SCLC, cervical cancers.

    • Antiviral/Antiparasitic Analysis: Rising exercise towards HIV-1, HSV, Leishmania in preclinical fashions.

    • (Be aware: Nattokinase primarily exhibits anticoagulant/thrombolytic results; direct kidney advantages are much less established vs. potential CPT nephrotoxicity issues).

  • Dosage & Administration:

    • NOT a dietary complement. Used clinically as IV derivatives below strict medical supervision.

    • Analysis doses differ (usually 0.1-100 µM 시험관 내에서; mg/kg ranges 생체 내). No secure each day consumption for self-administration exists.

  • Important Precautions & Aspect Results:

    • Extreme Toxicity: Myelosuppression (neutropenia, thrombocytopenia), extreme diarrhea (cholinergic & delayed), hemorrhagic cystitis, hepatotoxicity.

    • Lactone Instability: Speedy hydrolysis to inactive carboxylate at physiological pH reduces efficacy.

    • 금기사항: Being pregnant/lactation (teratogenic), pre-existing bone marrow melancholy, energetic infections.

    • 다음을 처리합니다. Cytotoxic – Requires PPE (gloves, masks, lab coat). Keep away from inhalation/pores and skin contact.

4. Shaanxi Zhonghong: Your Premier Camptothecin Supply

Shaanxi Zhonghong Investment Technology Co., Ltd. is a cGMP/ISO-certified chief in high-purity plant-derived APIs. With 28+ years in bioactive phytochemistry, we focus on:

  • 핵심 역량:

    • 과학적 우수성: 5개 대학 공동 연구실, 20개 이상의 특허 (incl. CPT stabilization tech), Unique Natural Compound Library.

    • Slicing-Edge Analytics: HPLC-PDA/ELSD (purity), UPLC-QTOF-MS (impurity profiling), Superconducting FT-NMR (construction ID), ICP-MS (중금속) GC-MS/MS (residual solvents). Purity >99%, exceeding USP by 20%.

    • 국제적 성취: 제공합니다 80개 이상의 국가 – Pharma, Biotech, Tutorial Analysis.

    • 수직적 통합: 지속 가능한 소싱 → GMP Extraction → pH-Managed Purification → 동결건조.

5. 엄격한 제품 사양

오염 물질 종류 매개변수 사양 제한 기술을 살펴보세요
살충제 클로르피리포스 ≤ 0.01mg/kg GC-MS/MS
시페르메트린 ≤ 0.05mg/kg GC-ECD
완전히 다른 살충제 ≤ 0.1 mg/kg (매) EU 2021/601
중금속 납 ≤ 1.0mg/kg ICP-MS(USP <232>)
처럼 ≤ 0.5mg/kg ICP-MS(USP <232>)
CD ≤ 0.5mg/kg ICP-MS(USP <232>)
Hg ≤ 0.1mg/kg CVAAS (USP <232>)
미생물학 TAMC ≤ 10³ CFU/g USP <61>
TYMC ≤ 10² CFU/g USP <61>
대장균 10g에는 없음 USP <62>
살모넬라균 종 Absent in 375g USP <62>
Important High quality Camptothecin Purity ≥ 99.0% (HPLC) HPLC-PDA(USP <621>)
Lactone Kind ≥ 95.0% pH-Particular HPLC
수분 함량 ≤ 2.0% Karl Fischer (USP <921>)
잔류 용매 Class 1/2 Solvents: Undetected GC-FID(ICH Q3C)

6. 우수한 제조 워크플로

  1. 지속 가능한 수확: Camptotheca acuminata cultivated below GACP tips.

  2. 선택적 추출: Ethanol/Water 또는 초임계 CO₂ extraction of bark/seeds.

  3. 주요 정화: 액체-액체 분배 (CH₂Cl₂/H₂O), adsorbent chromatography (SiO₂, polyamide).

  4. Lactone Stabilization: pH-controlled crystallization (pH 3.5-4.5) to maximise lactone yield.

  5. Last Purification: 분취용 HPLC (C18 column, MeOH/H₂O/TFA cell section).

  6. 건조: 동결건조 (-50°C, <10 Pa) to protect lactone ring & stability.

  7. QC 출시: 가득한 코에이 verifying identification, purity, lactone content material, contaminants.

7. Key Application Areas

  • Oncology Medicine: Precursor for Irinotecan (CPT-11), Topotecan, Belotecan.

  • Most cancers Analysis: Software compound for DNA harm response, 세포사멸, drug resistance research.

  • Drug Supply Techniques: Loaded into liposomes, nanoparticles, polymer conjugates to boost solubility/concentrating on.

  • Antiviral/Antiparasitic Improvement: Investigational agent for novel therapeutics.

8. cGMP 고품질 관리 프로토콜
산시성 중홍(Shaanxi Zhonghong)은 다음과 같은 직원을 고용합니다. tiered QC technique:

  • 신분증: FT-NMR (¹H/¹³C), HPLC-UV/PDA spectral match vs. USP reference customary.

  • Assay & Impurities: HPLC-ELSD/PDA quantifies CPT (≥99.0%) and detects ≤0.1% associated compounds (10-deacetylcamptothecin, camptothecin carboxylate).

  • Lactone Content: Validated pH-specific RP-HPLC methodology distinguishes lactone/carboxylate types.

  • Contaminants: ICP-MS for heavy metals (USP <232>), GC-MS/MS for 500+ pesticides (EU 2021/601), LC-MS/MS for mycotoxins, USP <61>/<62> for microbiology.

  • 안정: Actual-time & accelerated ICH Q1A(R2) research verify 24-month shelf-life at -20°C (desiccated, argon ambiance).

9. Safe Packaging & Logistics

  • 메이저 팩: Amber glass vials sealed below argon, full of desiccant.

  • 보조 팩: Vacuum-sealed aluminum luggage 내부에 UN-certified HDPE containers.

  • 저장: -20°C ± 5°C; defend from gentle/moisture.

  • 배달: International cold-chain logistics (-15°C to -25°C) by way of DHL FedEx UltraCold.

10. Mechanisms, Functions & Challenges

  • 기구: Traps Topo I-DNA cleavable complexes → Collision with replication forks → DSBs → ATR/Chk1 activation → p53-mediated apoptosis.

  • Innovation: 중홍의 PEGylated liposomal CPT (Patent: CN202210XXXXXX) enhances tumor concentrating on.

  • 분석 프런티어:

    • Novel Analogues: 7-Silylcamptothecins (improved lactone stability).

    • Mixture Therapies: With PARP inhibitors, immunotherapy.

    • Biomarkers: Predictive biomarkers for CPT sensitivity (ERCC1, Topo I ranges).

  • 과제: Lactone instability, myelosuppression, ABCG2-mediated drug resistance, tumor heterogeneity.

11. Steadily Requested Questions (FAQ)

  • Q1: Can I take Camptothecin as a complement?

    • A: Completely NOT. CPT is a potent cytotoxic compound with extreme unintended effects. Use solely below medical supervision in FDA-approved by-product types (e.g., Irinotecan) or for managed analysis.

  • Q2: Why is lactone kind important?

    • 에이: Solely the closed lactone ring binds Topo I. The open carboxylate kind is inactive and will trigger renal toxicity. Zhonghong ensures ≥95% lactone content material by way of lyophilization.

  • Q3: How does Camptothecin differ from Nattokinase for kidneys?

    • 에이: CPT carries nephrotoxicity dangers (tubular harm). Nattokinase, a fibrinolytic enzyme, lacks strong proof for direct kidney advantages. Neither is nephroprotective.

  • This autumn: What analytical strategies verify Zhonghong’s CPT purity?

    • 에이: HPLC-PDA/ELSD (purity), UPLC-QTOF-MS (impurities), FT-NMR (construction), pH-HPLC (lactone quantification), ICP-MS (heavy metals).

  • Q5: Do you supply Camptothecin derivatives?

    • 에이: Sure. GMP-grade 10-Hydroxycamptothecin, SN-38 (Irinotecan metabolite), customized synthesis obtainable.

12. The place to Purchase?
Supply research-grade & GMP Camptothecin:

13. 결론
Camptothecin stays a cornerstone of topoisomerase-targeted oncology. Its medical impression by way of semisynthetic derivatives underscores its irreplaceable function. Success hinges on securing ultra-pure, lactone-stabilized materials with verifiable traceability. Shaanxi Zhonghong delivers >99% HPLC purity CPT ~을 통해 특허받은 안정화 전문성, cGMP 준수, 그리고 unmatched analytical rigor – empowering drug discovery and superior therapeutics.

14. 참고문헌

  1. Wall ME, et al. (1966). J Am Chem Soc. 88:3888–3890. [DOI:10.1021/ja00968a057]

  2. Hsiang YH, et al. (1985). J Biol Chem. 260:14873–14878. [PMID:2997227]

  3. Pommier Y. (2006). Nat Rev Most cancers. 6:789–802. [DOI:10.1038/nrc1977]

  4. USP-NF Monograph: Camptothecin. USP44-NF39.

  5. ICH Q3C (R8): Residual Solvents. (2021).

  6. FDA Steerage: Irinotecan Label. (2023).

  7. Thomas CJ, et al. (2004). J Med Chem. 47:3972–3981. [DOI:10.1021/jm030660+]

  8. Du W, et al. (2022). Acta Pharm Sin B. 12:3006–3025. [DOI:10.1016/j.apsb.2022.03.011]

  9. GLS-010-IPHASE Research Protocol (NCT04501563).

  10. EMA Evaluation Report: Topotecan (2020). EMA/CHMP/89262/2020.

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