Unlocking the Potential of Isosteviol: Nature’s Zero-Calorie Sweetener Powerhouse | Supplier & Manufacturer

1. What is Isosteviol?

Isosteviol is a diterpenoid carboxylic acid derived from its precursor, steviol glycosides, the intensely candy compounds discovered within the leaves of the Stevia rebaudiana Bertoni plant. Via a selected acid hydrolysis course of, the glucose moieties of stevioside are cleaved off, yielding isosteviol. In contrast to its guardian glycosides, isosteviol itself is non-caloric however possesses solely delicate sweetness. Its significance lies primarily in its function as a key metabolic intermediate and, more and more, in its personal distinct and promising bioactive properties, making it a compound of serious curiosity in meals science, nutraceuticals, and pharmaceutical analysis.

2. Product Supply, Chemical Properties & Identifiers

• Supply: Synthesized by way of chemical modification (hydrolysis) of steviol glycosides extracted from Stevia rebaudiana leaves.

• Chemical Properties:

  • Chemical Name: ent-16-Ketobeyeran-19-oic acid or 13-Hydroxyent-kaur-16-en-19-oic acid

  • Molecular Components: C₂₀H₃₀O₃

  • Molecular Weight: 318.45 g/mol

  • Appearance: Usually a high quality, white to off-white crystalline powder.

  • Solubility: Reveals lipophilic traits, soluble in organic solvents like ethanol, methanol, chloroform, and dimethyl sulfoxide (DMSO), however displays low solubility in water.

  • Stability: Chemically secure beneath typical storage situations. Immune to warmth and pH adjustments encountered in meals processing.

• Key Identifiers:

  • CAS Registry Quantity: 471-80-7

  • EC Quantity (EINECS): 207-442-5

  • MF: C₂₀H₃₀O₃

  • MW: 318.45

3. Benefits, Purity, Dosage & Security Issues

• “Greatest” & Highest Purity: The “finest” isosteviol prioritizes distinctive purity (>98%), rigorous absence of residual solvents, and undetectable ranges of contaminants (heavy metals, pesticides, microbes). Respected suppliers like Shaanxi Zhonghong Investment Technology Co., Ltd. obtain this by means of proprietary purification strategies (e.g., multi-stage crystallization, preparative HPLC) exceeding normal {industry} purity benchmarks.

• Well being Benefits (Rising Analysis):

  • Cardiometabolic Well being: Exhibits potential in modulating glucose homeostasis (enhancing insulin sensitivity), managing dyslipidemia (decreasing triglycerides, LDL), and supporting wholesome blood strain by way of results on vascular tone and endothelial perform. Acts partly by means of AMPK activation and PPARγ modulation.

  • Anti-inflammatory & Antioxidant: Reveals important reactive oxygen species (ROS) scavenging skill and modulates key inflammatory pathways (e.g., NF-κB, MAPK signaling).

  • Renal Safety: Analysis suggests protecting results towards renal fibrosis and glomerular injury in experimental fashions, doubtlessly by way of TGF-β/Smad pathway inhibition and oxidative stress discount. (Word: Whereas nattokinase is researched for cardiovascular advantages, its direct affect on kidney well being is much less established in comparison with the rising renal protecting knowledge for isosteviol).

  • Neuroprotective Potential: Preliminary research point out attainable advantages in neurodegenerative situations by decreasing neuroinflammation and oxidative injury.

• Every day Consumption & Utilization Tips: At present, no established ADI (Acceptable Every day Consumption) exists particularly for isosteviol. Dosing in analysis varies considerably. Crucially seek the advice of a healthcare skilled earlier than supplementation. Utilization relies upon closely on the appliance (meals additive, complement, analysis chemical). Observe particular product labeling or analysis protocols meticulously.

• Precautions & Facet Results:

  • Restricted Human Security Information: Complete long-term human research are nonetheless wanted.

  • Potential Drug Interactions: Theoretical interactions exist because of results on CYP450 enzymes and blood strain/glucose; warning with antihypertensives, antidiabetics, and anticoagulants.

  • Being pregnant & Lactation: Keep away from use because of inadequate security knowledge.

  • Reported Facet Results: Typically well-tolerated in analysis, however excessive doses might doubtlessly trigger gastrointestinal discomfort. Allergic reactions are uncommon however attainable.

  • High quality is Paramount: Underscores the need of sourcing ultra-pure isosteviol from GMP-compliant producers to attenuate contaminant dangers.

4. Introducing Shaanxi Zhonghong Investment Technology Co., Ltd.: Your Trusted Isosteviol Associate

Shaanxi Zhonghong Investment Technology Co., Ltd. stands as a premier high-tech enterprise devoted to excellence in bioactive compound analysis, growth, and manufacturing. We specialize within the superior extraction, isolation, and purification of potent phytochemicals, serving world markets in nutraceuticals, prescribed drugs, cosmeceuticals, meals components (pure sweeteners, colours), and analysis chemical substances.

• Core Competencies & Differentiation:

  • Unmatched Scientific Prowess (The Analysis Barrier): Strategic academic-industry partnerships with 5 elite universities underpin our innovation. We possess a formidable IP portfolio exceeding 20 patents and keep a globally unique compound library, driving cutting-edge discovery.

  • Analytical Management (Gear Dominance): Our state-of-the-art QC infrastructure options Excessive-Efficiency Liquid Chromatography (HPLC/UHPLC), Gasoline Chromatography-Mass Spectrometry (GC-MS), Superconducting Fourier Rework Nuclear Magnetic Resonance (FT-NMR), and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). This allows stringent purity verification (>98% normal, exceeding {industry} norms by 20%) and complete contaminant profiling.

  • International Provide Chain Excellence: We reliably serve a various clientele throughout 80+ nations all through Asia, Europe, and the Americas, offering customized synthesis and bespoke ingredient options to multinational pharmaceutical companies, analysis establishments, and complement manufacturers.

  • Legacy of Experience: Leveraging 28 years of specialised expertise within the isolation and software of bioactive pure compounds.

5. Product Specs: Rigorous High quality Assurance

6. Superior Manufacturing Course of
Our isosteviol manufacturing employs a vertically built-in, GMP-compliant workflow:

1. Sourcing & Authentication: Premium Stevia rebaudiana leaves bear botanical verification (macroscopic/microscopic, DNA barcoding).

2. Main Extraction: Supercritical CO2 extraction or food-grade solvent extraction yields crude steviol glycosides.

3. Hydrolysis: Managed acid-catalyzed hydrolysis selectively cleaves glycosidic bonds, changing stevioside/rebaudioside A to isosteviol.

4. Purification: Multi-stage liquid-liquid extraction, adopted by activated carbon decolorization and multi-step fractional crystallization beneath exact temperature management. Preparative HPLC is employed for ultra-high purity (>99%) batches.

5. Drying & Milling: Lyophilization (freeze-drying) or vacuum belt drying ensures stability, adopted by micronization to desired particle measurement distribution.

6. Strict High quality Management: Each batch undergoes complete analytical profiling towards stringent specs earlier than launch.

7. Numerous Application Scenarios

• Nutraceuticals & Dietary Dietary supplements: Formulated in capsules, tablets, softgels, and powders concentrating on metabolic well being, cardiovascular assist, antioxidant protection, and anti-aging.

• Practical Meals & Drinks: Integrated as a non-caloric practical ingredient in health-focused drinks, snacks, and meal replacements, leveraging its bioactivity past sweetness.

• Pharmaceutical Analysis: An important reference normal, pharmacophore, and lead compound in drug discovery for diabetes, hypertension, irritation, and neurodegeneration.

• Cosmeceuticals: Explored in topical formulations for anti-aging (combating oxidative stress/irritation) and skin-brightening results.

• Sweetener Intermediate: Key precursor within the synthesis of next-generation steviol glycosides with improved style profiles.

8. Stringent High quality Management (QC)
High quality is embedded at each stage. Our ISO 9001 and cGMP-aligned QC system mandates:

• Identification Affirmation: FT-NMR and HPLC-UV/ELSD fingerprint matching towards reference requirements.

• Purity & Efficiency: HPLC-ELSD/UV quantification of isosteviol content material (≥98%), monitoring for associated compounds (steviol, steviol glycosides).

• Contaminant Management: GC-MS/MS/LC-MS/MS screens for >500 pesticide residues. ICP-MS ensures heavy metals compliance. Microbiological testing (cardio depend, pathogens) follows validated USP/EP strategies.

• Residual Solvents: GC-FID/HS-GC evaluation per ICH Q3C pointers.

• Bodily & Chemical Assessments: Loss on Drying (LOD), Residue on Ignition (Sulfated Ash), pH, Particular Rotation.

• Stability Research: Ongoing ICH Q1A(R2) compliant accelerated and long-term stability research to outline shelf life and storage situations. Documentation & Traceability: Full Certificates of Evaluation (CoA) per batch, adhering to ALCOA+ rules for knowledge integrity.

9. Safe Packaging & International Logistics

• Packaging: Meals-grade double HDPE baggage with integrity liners, sealed inside strong, tamper-evident fiber drums (25kg normal). Smaller R&D portions use glass bottles. Customized packaging (vacuum-sealed, nitrogen-flushed) out there.

• Storage: Retailer in a cool, dry place (<25°C), protected against gentle and moisture. Preserve unique sealed packaging.

• Logistics: Environment friendly world delivery by way of air freight, sea freight, or categorical couriers. Experience in dealing with customs clearance and regulatory documentation for worldwide markets. Chilly chain choices out there.

10. Well being Efficacy, Mechanism & Analysis

• Mechanism Insights: Isosteviol exerts results by way of a number of pathways:

  • AMPK Activation: Enhances glucose uptake, fatty acid oxidation.

  • PPARγ Modulation: Influences adipogenesis, insulin sensitivity, irritation.

  • Okay+ Channel Activation (Vascular): Promotes vasodilation, reducing blood strain.

  • TGF-β1/Smad Pathway Inhibition: Key mechanism in decreasing renal fibrosis.

  • NF-κB & MAPK Suppression: Reduces pro-inflammatory cytokine manufacturing (TNF-α, IL-6).

  • ROS Scavenging: Direct antioxidant exercise and upregulation of endogenous antioxidants (SOD, GSH).

• Business Purposes & Innovation: Driving innovation in practical sweeteners, metabolic syndrome therapeutics, pure preservatives, and cosmeceutical actives. Zhonghong’s patented enzymatic bioconversion and steady chromatography applied sciences improve yield and purity.

• Analysis Frontiers & Challenges:

  • Frontiers: Exploring isosteviol derivatives for enhanced bioavailability/exercise; medical translation of preclinical advantages; mixture therapies.

  • Challenges: Establishing human-specific ADI; definitive large-scale human medical trials; optimizing supply methods for poor water solubility; navigating advanced world regulatory landscapes (GRAS, Novel Meals, and so forth.).

11. Often Requested Questions (FAQ)

• Q1: Is Isosteviol the identical as Stevia?

  • A: No. Stevia refers back to the plant or its candy leaf extracts (steviol glycosides like stevioside, rebaudioside A). Isosteviol is a particular metabolite/spinoff produced by chemically modifying these glycosides. It has completely different properties (mildly candy/non-sweet, distinct bioactivity).

• Q2: Is Isosteviol secure?

  • A: Present preclinical and restricted human knowledge counsel security profile at researched doses. Nonetheless, long-term, large-scale human research are ongoing. All the time select high-purity (>98%) isosteviol from respected suppliers adhering to GMP and seek the advice of your healthcare supplier earlier than use, particularly if pregnant, breastfeeding, taking treatment, or have underlying situations.

• Q3: What are the primary well being advantages of Isosteviol?

  • A: Analysis highlights potential for supporting wholesome blood sugar metabolism, cardiovascular perform (blood strain, lipids), decreasing irritation and oxidative stress, and defending kidney well being. Extra human trials are wanted to verify efficacy for particular situations.

• This autumn: What is the really useful each day dosage of Isosteviol?

  • A: There may be no universally established each day consumption (ADI). Dosages utilized in analysis fluctuate broadly. Crucially, seek the advice of your physician or a certified healthcare practitioner for customized recommendation primarily based in your well being targets and circumstances. Observe product-specific labeling.

• Q5: How does Isosteviol examine to Nattokinase for kidney well being?

  • A: Whereas each are researched for well being advantages, their mechanisms differ. Isosteviol reveals direct anti-fibrotic and antioxidant results in kidney tissue in preclinical fashions, primarily by way of TGF-β inhibition. Nattokinase is a fibrinolytic enzyme primarily studied for cardiovascular advantages (blood move, clot prevention); its direct affect on kidney construction/perform is much less established. Neither replaces medical therapy for kidney illness.

• Q6: Why select Zhonghong’s Isosteviol?

  • A: Zhonghong delivers ultra-high purity (>98%), industry-leading contaminant management, batch-to-batch consistency, backed by 20+ patents, cutting-edge analytical capabilities (HPLC, NMR, ICP-MS), GMP compliance, and 28 years of bioactive compound experience. We provide customization and world regulatory assist.

12. The place to Purchase Excessive-Purity Isosteviol?
Supply premium Isosteviol instantly from the producer:

• Web site: Discover merchandise and specs at aiherba.com

• Contact: Attain our gross sales staff by way of e-mail: liaodaohai@gmail.com

• Inquiry: Request an in depth Certificates of Evaluation (CoA), MSDS, and aggressive citation on your particular wants (bulk, analysis samples).

13. Conclusion: Isosteviol – A Versatile Bioactive with Important Promise
Isosteviol, derived from the pure sweetener stevia, transcends its origins as a metabolic intermediate. Its compelling portfolio of bioactivities – spanning cardiometabolic regulation, renoprotection, anti-inflammation, and antioxidant protection – positions it as a extremely promising compound for the nutraceutical, practical meals, and pharmaceutical industries. Whereas additional strong human medical validation is crucial, the prevailing scientific basis is powerful. The vital issue for efficacy and security lies in sourcing ultra-pure, meticulously characterised isosteviol. Shaanxi Zhonghong Investment Technology Co., Ltd., with its scientific rigor, superior purification know-how, and unwavering dedication to high quality, stands because the premier world accomplice for unlocking the complete potential of this exceptional diterpenoid. Contact us right this moment to debate your isosteviol necessities.

14. References

1. Wang, J., et al. (2014). Isosteviol reduces plasma glucose ranges in diabetic Goto-Kakizaki rats by enhancing insulin sensitivity and enhancing glucose metabolism. European Journal of Pharmacology, 734, 50-55. DOI: 10.1016/j.ejphar.2014.03.036

2. Liu, J. C., et al. (2010). Antihypertensive results of isosteviol in spontaneously hypertensive rats. Molecular and Mobile Biochemistry, 340(1-2), 55-60. DOI: 10.1007/s11010-010-0401-1

3. Mizushina, Y., et al. (2005). Structural evaluation of isosteviol and associated compounds as DNA polymerase and DNA topoisomerase inhibitors. Life Sciences, 77(17), 2127-2140. DOI: 10.1016/j.lfs.2005.03.022

4. Chen, T. H., et al. (2016). Isosteviol protects towards endotoxin-induced acute kidney harm by inhibiting NF-κB and MAPK signaling pathways. Journal of Agricultural and Meals Chemistry, 64(34), 6669-6677. DOI: 10.1021/acs.jafc.6b02895

5. Worldwide Stevia Council. (2023). Stevia Manufacturing & Science. https://internationalsteviacouncil.org/

6. EFSA Panel on Meals Components and Flavourings (FAF). (2021). Security of use of steviol glycosides. EFSA Journal, 19(1), e06336. DOI: 10.2903/j.efsa.2021.6336

7. FDA GRAS Notices: GRN No. 252 (Steviol Glycosides), GRN No. 667 (Reb M). https://www.fda.gov/food/gras-notice-inventory

8. JECFA (Joint FAO/WHO Skilled Committee on Meals Components). (2016). Steviol glycosides. WHO Meals Components Sequence 71. https://apps.who.int/food-additives-contaminants-jecfa-database/

9. ICH High quality Tips (Q1A(R2), Q2(R1), Q3C). https://www.ich.org/page/quality-guidelines

10. USP-NF Normal Chapters: <61> Microbiological Examination, <62> Microbiological Procedures, <731> Loss on Drying, <281> Residue on Ignition. https://www.usp.org/