Unlocking the Potential of Isosteviol: Nature’s Zero-Calorie Sweetener Powerhouse

1. What is Isosteviol?

Isosteviol is a diterpenoid carboxylic acid derived from its precursor, steviol glycosides, the intensely sweet compounds found in the leaves of the ষ্টেভিয়া ৰেবাউডিয়ানা Bertoni plant. Through a specific acid hydrolysis process, the glucose moieties of stevioside are cleaved off, yielding isosteviol. Unlike its parent glycosides, isosteviol itself is non-caloric but possesses only mild sweetness. Its significance lies primarily in its role as a key metabolic intermediate and, increasingly, in its own distinct and promising জৈৱ সক্ৰিয় ধৰ্ম, making it a compound of significant interest in food science, nutraceuticals, and pharmaceutical research.

২/ পণ্যৰ উৎস, ৰাসায়নিক ধৰ্ম আৰু চিনাক্তকৰ্তা

• উত্‍স: Synthesized via chemical modification (hydrolysis) of steviol glycosides extracted from ষ্টেভিয়া ৰেবাউডিয়ানা leaves.

• ৰাসায়নিক ধৰ্ম:

  • Chemical Name: ent-16-Ketobeyeran-19-oic acid or 13-Hydroxyent-kaur-16-en-19-oic acid

  • আণৱিক সূত্ৰ: C₂₀H₃₀O₃

  • আণৱিক ওজন: 318.45 g/mol

  • ৰূপ: Typically a fine, white to off-white crystalline powder.

  • দ্ৰৱণীয়তা: প্ৰদৰ্শনী lipophilic characteristics, soluble in organic solvents like ethanol, methanol, chloroform, and dimethyl sulfoxide (DMSO), but exhibits low solubility in water.

  • স্থিৰতা: Chemically stable under typical storage conditions. Resistant to heat and pH changes encountered in food processing.

• মূল চিনাক্তকৰ্তাসমূহ:

  • CAS পঞ্জীয়ন নম্বৰ: 471-80-7

  • ইচি নম্বৰ (EINECS): 207-442-5

  • এম এফ: C₂₀H₃₀O₃

  • মেগাৱাট: 318.45

3. Benefits, Purity, Dosage & Safety Considerations

• “Best” & Highest Purity: The “best” isosteviol prioritizes exceptional purity (>98%), rigorous absence of residual solvents, আৰু undetectable levels of contaminants (heavy metals, pesticides, microbes). Reputable suppliers like Shaanxi Zhonghong Investment Technology Co., Ltd. achieve this through proprietary purification techniques (e.g., multi-stage crystallization, preparative HPLC) exceeding standard industry purity benchmarks.

• Health Benefits (Emerging Research):

  • Cardiometabolic Health: Shows potential in modulating glucose homeostasis (improving insulin sensitivity), managing dyslipidemia (reducing triglycerides, LDL), and supporting healthy blood pressure via effects on vascular tone and endothelial function. Acts partly through AMPK সক্ৰিয়কৰণ আৰু PPARγ modulation.

  • এন্টি-ইনফ্লেমেটৰী আৰু এন্টিঅক্সিডেন্ট: Exhibits significant reactive oxygen species (ROS) scavenging ability and modulates key inflammatory pathways (e.g., NF-κB, MAPK signaling).

  • Renal Protection: Research suggests protective effects against renal fibrosis আৰু glomerular damage in experimental models, potentially via TGF-β/Smad pathway inhibition আৰু oxidative stress reduction. (Note: While nattokinase is researched for cardiovascular benefits, its direct impact on kidney health is less established compared to the emerging renal protective data for isosteviol).

  • স্নায়ুৰক্ষামূলক সম্ভাৱনা: Preliminary studies indicate possible benefits in neurodegenerative conditions by reducing neuroinflammation and oxidative damage.

• Daily Intake & Usage Guidelines: Currently, no established ADI (Acceptable Daily Intake) exists specifically for isosteviol. Dosing in research varies significantly. Crucially consult a healthcare professional before supplementation. Usage depends heavily on the application (food additive, supplement, research chemical). Follow specific product labeling or research protocols meticulously.

• সাৱধানতা আৰু পাৰ্শ্বক্ৰিয়া:

  • Limited Human Safety Data: Comprehensive long-term human studies are still needed.

  • Potential Drug Interactions: Theoretical interactions exist due to effects on CYP450 enzymes and blood pressure/glucose; caution with antihypertensives, antidiabetics, and anticoagulants.

  • Pregnancy & Lactation: Avoid use due to insufficient safety data.

  • Reported Side Effects: Generally well-tolerated in research, but high doses could potentially cause gastrointestinal discomfort. Allergic reactions are rare but possible.

  • গুণগত মান সৰ্বোচ্চ: Underscores the necessity of sourcing ultra-pure isosteviol from GMP-compliant manufacturers to minimize contaminant risks.

4. Introducing Shaanxi Zhonghong Investment Technology Co., Ltd.: Your Trusted Isosteviol Partner

Shaanxi Zhonghong Investment Technology Co., Ltd. stands as a premier উচ্চ প্ৰযুক্তিৰ উদ্যোগ dedicated to excellence in জৈৱ সক্ৰিয় যৌগ research, development, and manufacturing. We specialize in the advanced নিষ্কাশন, পৃথকীকৰণ, আৰু বিশুদ্ধকৰণ of potent phytochemicals, serving global markets in nutraceuticals, pharmaceuticals, cosmeceuticals, food additives (natural sweeteners, colors), and research chemicals.

• মূল দক্ষতা আৰু পাৰ্থক্য:

  • Unmatched Scientific Prowess (The Research Barrier): কৌশলগত academic-industry partnerships with 5 elite universities underpin our innovation. We possess a formidable IP portfolio exceeding 20 patents and maintain a বিশ্বব্যাপী একচেটিয়া যৌগ লাইব্ৰেৰী, driving cutting-edge discovery.

  • Analytical Leadership (Equipment Dominance): আমাৰ state-of-the-art QC infrastructure features High-Performance Liquid Chromatography (HPLC/UHPLC), Gas Chromatography-Mass Spectrometry (GC-MS), Superconducting Fourier Transform Nuclear Magnetic Resonance (FT-NMR), আৰু ইণ্ডাক্টিভলি কাপল্ড প্লাজমা মাছ স্পেকট্ৰ'মেট্ৰি (ICP-MS). ই সক্ষম কৰে stringent purity verification (>98% standard, exceeding industry norms by 20%) and comprehensive contaminant profiling.

  • Global Supply Chain Excellence: We reliably serve a diverse clientele across ৮০+ দেশ throughout Asia, Europe, and the Americas, providing custom synthesis আৰু বেচপক উপাদান সমাধান to multinational pharmaceutical corporations, research institutions, and supplement brands.

  • Legacy of Expertise: Leveraging ২৮ বছৰৰ বিশেষ অভিজ্ঞতা in the isolation and application of bioactive natural compounds.

5. Product Specifications: Rigorous Quality Assurance

6. Advanced Production Process
Our isosteviol production employs a vertically integrated, GMP-compliant workflow:

1. উৎস আৰু প্ৰমাণীকৰণ: প্ৰিমিয়াম ষ্টেভিয়া ৰেবাউডিয়ানা leaves undergo botanical verification (macroscopic/microscopic, DNA barcoding).

2. প্ৰাথমিক নিষ্কাশন: Supercritical CO2 extraction অথবা food-grade solvent extraction yields crude steviol glycosides.

3. Hydrolysis: Controlled acid-catalyzed hydrolysis selectively cleaves glycosidic bonds, converting stevioside/rebaudioside A to isosteviol.

4. বিশুদ্ধকৰণ: Multi-stage liquid-liquid extraction, followed by activated carbon decolorization আৰু multi-step fractional crystallization under precise temperature control. প্ৰস্তুতিমূলক এইচ পি এল চি is employed for ultra-high purity (>99%) batches.

5. Drying & Milling: Lyophilization (freeze-drying) অথবা vacuum belt drying ensures stability, followed by micronization to desired particle size distribution.

6. কঠোৰ গুণগত নিয়ন্ত্ৰণ: প্ৰতিটো বেচৰ মাজেৰে পাৰ হয় comprehensive analytical profiling against stringent specifications before release.

৭/ বৈচিত্ৰময় প্ৰয়োগৰ পৰিস্থিতি

• নিউট্ৰাচিউটিকেলছ আৰু খাদ্য পৰিপূৰক: Formulated in capsules, tablets, softgels, and powders targeting metabolic health, cardiovascular support, antioxidant defense, and anti-aging.

• কাৰ্য্যকৰী খাদ্য আৰু পানীয়: Incorporated as a non-caloric functional ingredient in health-focused drinks, snacks, and meal replacements, leveraging its bioactivity beyond sweetness.

• Pharmaceutical Research: A crucial reference standard, pharmacophore, আৰু lead compound in drug discovery for diabetes, hypertension, inflammation, and neurodegeneration.

• প্ৰসাধন সামগ্ৰী: Explored in topical formulations for বয়স বৃদ্ধি বিৰোধী (combating oxidative stress/inflammation) and skin-brightening প্ৰভাৱ।

• Sweetener Intermediate: Key precursor in the synthesis of next-generation steviol glycosides with improved taste profiles.

৮) কঠোৰ গুণগত নিয়ন্ত্ৰণ (QC)
Quality is embedded at every stage. Our ISO 9001 and cGMP-aligned QC system mandates:

• Identity Confirmation: FT-NMR আৰু এইচ পি এল চি-ইউভি/ই এল এছ ডি fingerprint matching against reference standards.

• Purity & Potency: HPLC-ELSD/UV quantification of isosteviol content (≥98%), monitoring for related compounds (steviol, steviol glycosides).

• Contaminant Control: GC-MS/MS/LC-MS/MS screens for >500 pesticide residues. আইচিপি-এমএছ ensures heavy metals compliance. অণুজীৱবিজ্ঞান পৰীক্ষা (aerobic count, pathogens) follows validated USP/EP methods.

• অৱশিষ্ট দ্রাৱক: জিচি-এফআইডি/এইচএছ-জিচি analysis per আইচিএইচ কিউ৩চি guidelines.

• Physical & Chemical Tests: Loss on Drying (LOD), প্ৰজ্বলনৰ সময়ত অৱশিষ্ট (ছালফেটেড এছ), pH, Specific Rotation.

• স্থিতিশীলতা অধ্যয়ন: Ongoing আইচিএইচ কিউ১এ(আৰ২) compliant accelerated আৰু long-term stability studies to define shelf life and storage conditions. নথিপত্ৰ আৰু অনুসৰণযোগ্যতা: সম্পূৰ্ণ Certificate of Analysis (CoA) per batch, adhering to ALCOA+ principles for data integrity.

৯) সুৰক্ষিত পেকেজিং আৰু গ্ল’বেল লজিষ্টিক

• পেকেজিং: খাদ্য-গ্ৰেড double HDPE bags with integrity liners, sealed inside robust, tamper-evident fiber drums (25kg standard). Smaller R&D quantities use glass bottles. Custom packaging (vacuum-sealed, nitrogen-flushed) available.

• সংৰক্ষণ: ষ্ট'ৰ কৰক ক cool, dry place (<25°C), protected from light and moisture. Maintain original sealed packaging.

• লজিষ্টিক: Efficient global shipping via air freight, sea freight, or express couriers. Expertise in handling customs clearance আৰু regulatory documentation for international markets. Cold chain options available.

10. Health Efficacy, Mechanism & Research

• Mechanism Insights: Isosteviol exerts effects via multiple pathways:

  • AMPK Activation: Enhances glucose uptake, fatty acid oxidation.

  • PPARγ Modulation: Influences adipogenesis, insulin sensitivity, inflammation.

  • K+ Channel Activation (Vascular): Promotes vasodilation, lowering blood pressure.

  • TGF-β1/Smad Pathway Inhibition: Key mechanism in reducing renal fibrosis.

  • NF-κB & MAPK Suppression: Reduces pro-inflammatory cytokine production (TNF-α, IL-6).

  • ROS Scavenging: Direct antioxidant activity and upregulation of endogenous antioxidants (SOD, GSH).

• উদ্যোগ প্ৰয়োগ আৰু উদ্ভাৱন: Driving innovation in functional sweeteners, metabolic syndrome therapeutics, natural preservatives, আৰু cosmeceutical actives. Zhonghong’s patented enzymatic bioconversion আৰু continuous chromatography technologies enhance yield and purity.

• গৱেষণাৰ সীমা আৰু প্ৰত্যাহ্বানসমূহ:

  • সীমান্ত: অন্বেষণ কৰা isosteviol derivatives for enhanced bioavailability/activity; clinical translation of preclinical benefits; combination therapies.

  • প্ৰত্যাহ্বানসমূহ: Establishing human-specific ADI; definitive large-scale human clinical trials; optimizing delivery systems for poor water solubility; navigating complex global regulatory landscapes (GRAS, Novel Food, etc.).

১১) সঘনাই সোধা প্ৰশ্ন (FAQ)

• Q1: Is Isosteviol the same as Stevia?

  • উঃ: No. Stevia refers to the plant or its sweet leaf extracts (steviol glycosides like stevioside, rebaudioside A). Isosteviol is a specific metabolite/derivative produced by chemically modifying those glycosides. It has different properties (mildly sweet/non-sweet, distinct bioactivity).

• Q2: Is Isosteviol safe?

  • উঃ: Existing preclinical and limited human data suggest a good safety profile at researched doses. However, long-term, large-scale human studies are ongoing. Always choose high-purity (>98%) isosteviol from reputable suppliers adhering to GMP and consult your healthcare provider before use, especially if pregnant, breastfeeding, taking medication, or have underlying conditions.

• Q3: What are the main health benefits of Isosteviol?

  • উঃ: Research highlights potential for supporting healthy blood sugar metabolism, cardiovascular function (blood pressure, lipids), reducing inflammation and oxidative stress, and protecting kidney health. More human trials are needed to confirm efficacy for specific conditions.

• Q4: What is the recommended daily dosage of Isosteviol?

  • উঃ: There is no universally established daily intake (ADI). Dosages used in research vary widely. Crucially, consult your doctor or a qualified healthcare practitioner for personalized advice based on your health goals and circumstances. Follow product-specific labeling.

• Q5: How does Isosteviol compare to Nattokinase for kidney health?

  • উঃ: While both are researched for health benefits, their mechanisms differ. Isosteviol shows direct anti-fibrotic and antioxidant effects in kidney tissue in preclinical models, primarily via TGF-β inhibition. Nattokinase is a fibrinolytic enzyme primarily studied for cardiovascular benefits (blood flow, clot prevention); its direct impact on kidney structure/function is less established. Neither replaces medical treatment for kidney disease.

• Q6: Why choose Zhonghong’s Isosteviol?

  • উঃ: Zhonghong delivers ultra-high purity (>98%), industry-leading contaminant control, batch-to-batch consistency, backed by 20+ patents, cutting-edge analytical capabilities (HPLC, NMR, ICP-MS), GMP compliance, আৰু ২৮ বছৰীয়া জৈৱ সক্ৰিয় যৌগ বিশেষজ্ঞতা. We offer customization আৰু global regulatory support.

12. Where to Buy High-Purity Isosteviol?
Source premium Isosteviol directly from the manufacturer:

• ৱেবছাইট: Explore products and specifications at aiherba.com

• যোগাযোগ কৰক: Reach our sales team via email: liaodaohai@gmail.com ত যোগাযোগ কৰক

• Inquiry: Request a detailed Certificate of Analysis (CoA), MSDS, আৰু competitive quotation for your specific needs (bulk, research samples).

13. Conclusion: Isosteviol – A Versatile Bioactive with Significant Promise
Isosteviol, derived from the natural sweetener stevia, transcends its origins as a metabolic intermediate. Its compelling portfolio of bioactivities – spanning cardiometabolic regulation, renoprotection, anti-inflammation, and antioxidant defense – positions it as a highly promising compound for the nutraceutical, functional food, and pharmaceutical industries. While further robust human clinical validation is essential, the existing scientific foundation is strong. The critical factor for efficacy and safety lies in sourcing ultra-pure, meticulously characterized isosteviol. Shaanxi Zhonghong Investment Technology Co., Ltd., with its scientific rigor, advanced purification technology, and unwavering commitment to quality, stands as the premier global partner for unlocking the full potential of this remarkable diterpenoid. Contact us today to discuss your isosteviol requirements.

১৪) তথ্যসূত্ৰ

1. Wang, J., et al. (2014). Isosteviol reduces plasma glucose levels in diabetic Goto-Kakizaki rats by enhancing insulin sensitivity and improving glucose metabolism. ইউৰোপিয়ান জাৰ্নেল অৱ ফাৰ্মাক’লজি, 734, 50-55. DOI: 10.1016/j.ejphar.2014.03.036

2. Liu, J. C., et al. (2010). Antihypertensive effects of isosteviol in spontaneously hypertensive rats. Molecular and Cellular Biochemistry, 340(1-2), 55-60. DOI: 10.1007/s11010-010-0401-1

3. Mizushina, Y., et al. (2005). Structural analysis of isosteviol and related compounds as DNA polymerase and DNA topoisomerase inhibitors. জীৱন বিজ্ঞান, 77(17), 2127-2140. DOI: 10.1016/j.lfs.2005.03.022

4. Chen, T. H., et al. (2016). Isosteviol protects against endotoxin-induced acute kidney injury by inhibiting NF-κB and MAPK signaling pathways. কৃষি আৰু খাদ্য ৰসায়ন বিজ্ঞানৰ জাৰ্নেল, 64(34), 6669-6677. DOI: 10.1021/acs.jafc.6b02895

5. International Stevia Council. (2023). Stevia Production & Science. https://internationalsteviacouncil.org/

6. EFSA Panel on Food Additives and Flavourings (FAF). (2021). Safety of use of steviol glycosides. ই এফ এছ এ জাৰ্নেল, 19(1), e06336. DOI: 10.2903/j.efsa.2021.6336

7. FDA GRAS Notices: GRN No. 252 (Steviol Glycosides), GRN No. 667 (Reb M). https://www.fda.gov/food/gras-notice-inventory

8. JECFA (Joint FAO/WHO Expert Committee on Food Additives). (2016). Steviol glycosides. WHO Food Additives Series 71. https://apps.who.int/food-additives-contaminants-jecfa-database/

9. ICH Quality Guidelines (Q1A(R2), Q2(R1), Q3C). https://www.ich.org/page/quality-guidelines

10. USP-NF General Chapters: <61> Microbiological Examination, <62> Microbiological Procedures, <731> Loss on Drying, <281> Residue on Ignition. https://www.usp.org/